ELISA Kit for Complement Factor H Related Protein 1 (CFHR1)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.625-40ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.269ng/mL

Sample type

Serum, plasma and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Factor H Related Protein 1 (CFHR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Factor H Related Protein 1 (CFHR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Factor H Related Protein 1 (CFHR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complement Factor H Related Protein 1 (CFHR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

CFHL; CFHL1; CFHL1P; CFHR1P; FHR1; H36-1; H36-2; HFL1; HFL2; H factor-like protein 1

Item Name

Complement Factor H Related Protein 1

Research Area

Hematology;

Reference

IL‑6 and IL‑8 enhance factor H binding to the cell membranes;The influence of insulin resistance on cerebrospinal fluid and plasma biomarkers of Alzheimer’s pathology;Quantitative analysis of hydroxyapatite-binding plasma proteins in genotyped individuals with late-stage age-related macular degeneration;