ELISA Kit for N-Terminal Pro-C-Type Natriuretic Peptide (NT-ProCNP)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

12.35-1,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 5.32pg/mL

Sample type

Serum, plasma and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to N-Terminal Pro-C-Type Natriuretic Peptide (NT-ProCNP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled N-Terminal Pro-C-Type Natriuretic Peptide (NT-ProCNP) and unlabeled N-Terminal Pro-C-Type Natriuretic Peptide (NT-ProCNP) (Standards or samples) with the pre-coated antibody specific to N-Terminal Pro-C-Type Natriuretic Peptide (NT-ProCNP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of N-Terminal Pro-C-Type Natriuretic Peptide (NT-ProCNP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of N-Terminal Pro-C-Type Natriuretic Peptide (NT-ProCNP) in the sample.

Alternative Names

N-CNP

Item Name

N-Terminal Pro-C-Type Natriuretic Peptide

Research Area

Reference

Effects of fluoride on insulin signaling and bone metabolism in ovariectomized rats.;Modulating the phenotype of host macrophages to enhance osteogenesis in MSC-laden hydrogels: Design of a glucomannan coating material.;