High Sensitive ELISA Kit for C Reactive Protein (CRP)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

62.5-4,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 23.3pg/mL

Sample type

serum, plasma, tissue homogenates, cell lysates, urine, cerebrospinal fluid, cell culture supernates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive C Reactive Protein (CRP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive C Reactive Protein (CRP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive C Reactive Protein (CRP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of High Sensitive C Reactive Protein (CRP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

C-RP; PTX1; Pentraxin-Related; Pentraxin 1

Item Name

C Reactive Protein

Research Area

Tumor immunity;Infection immunity;Cardiovascular biology;Rheumatology;

Reference

Olive (Olea europaea L.) Leaf Polyphenols Improve Insulin Sensitivity in Middle-Aged Overweight Men: A Randomized, Placebo-Controlled, Crossover Trial;Relationships among serum C-reactive protein, receptor for advanced glycation products, metabolic dysfunction, and cognitive impairments;Among overweight middle-aged men, first-borns have lower insulin sensitivity than second-borns;Effect of caffeic acid on doxorubicin induced cardiotoxicity in rats;Effect of high free fatty acids on the anti-contractile response of perivascular adipose tissue in rat aorta;Anti-atherosclerosis and cardio-protective effects of the Angong Niuhuang Pill on a high fat and vitamin D3 induced rodent model of atherosclerosis.;The effects of non-surgical periodontal treatment on glycemic control, oxidative stress balance and quality of life in patients with type 2 diabetes: A randomized clinical trial;