ELISA Kit for Actin Alpha 2, Smooth Muscle (ACTa2)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

7.8-500ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 3.3ng/mL

Sample type

Tissue homogenates, cell lysates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Actin Alpha 2, Smooth Muscle (ACTa2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Actin Alpha 2, Smooth Muscle (ACTa2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Actin Alpha 2, Smooth Muscle (ACTa2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Actin Alpha 2, Smooth Muscle (ACTa2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

ACT-A2; ACTSA; ACTVS; Asma; GIG46; Cell growth-inhibiting gene 46 protein

Item Name

Actin Alpha 2, Smooth Muscle

Research Area

Developmental science;Bone metabolism;

Reference

Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonica;