ELISA Kit for Hemoglobin (HB)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

6.17-500ug/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 2.59ug/mL

Sample type

Serum, plasma, tissue homogenates, erythrocyte lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Hemoglobin (HB) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Hemoglobin (HB) and unlabeled Hemoglobin (HB) (Standards or samples) with the pre-coated antibody specific to Hemoglobin (HB). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Hemoglobin (HB) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Hemoglobin (HB) in the sample.

Alternative Names

Hgb; Haemoglobin; Heterotetramer(αβ)2

Item Name

Hemoglobin

Research Area

Metabolic pathway;Infection immunity;Hematology;Neuro science;

Reference

Chicory inulin ameliorates type 2 diabetes mellitus and suppresses JNK and MAPK pathways in vivo and in vitro;新規人工酸素運搬体HemoAct を用いた脳虚血再灌流障害治療開発に関する研究;