ELISA Kit for Luteinizing Hormone (LH)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

370.4-30,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Rhesus monkey (Simian)

Sensitivity

The minimum detectable dose of this kit is typically less than 137.2pg/mL

Sample type

Serum, plasma and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Luteinizing Hormone (LH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Luteinizing Hormone (LH) and unlabeled Luteinizing Hormone (LH) (Standards or samples) with the pre-coated antibody specific to Luteinizing Hormone (LH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Luteinizing Hormone (LH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Luteinizing Hormone (LH) in the sample.

Alternative Names

ICSH; Lutropin; Interstitial Cell Stimulating Hormone

Item Name

Luteinizing Hormone

Research Area

Endocrinology;Reproductive science;Hormone metabolism;

Reference