ELISA Kit for Phospholipase A2, Group V (PLA2G5)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

78-5000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 33pg/mL

Sample type

serum, plasma, tissue homogenates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase A2, Group V (PLA2G5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipase A2, Group V (PLA2G5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipase A2, Group V (PLA2G5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phospholipase A2, Group V (PLA2G5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

PLA2-10; Calcium-dependent phospholipase A2; Group V phospholipase A2; Phosphatidylcholine 2-acylhydrolase 5

Item Name

Phospholipase A2, Group V

Research Area

Enzyme & Kinase;

Reference