ELISA Kit for Cathelicidin Antimicrobial Peptide (CAMP)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.78-50ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Mus musculus (Mouse)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.28ng/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cathelicidin Antimicrobial Peptide (CAMP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cathelicidin Antimicrobial Peptide (CAMP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cathelicidin Antimicrobial Peptide (CAMP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cathelicidin Antimicrobial Peptide (CAMP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

CAP18; FALL39; HSD26; LL37; 18 kDa Cationic Antimicrobial Protein; Antibacterial protein FALL-39; FALL-39 peptide antibiotic

Item Name

Cathelicidin Antimicrobial Peptide

Research Area

Infection immunity;

Reference

Light-emitting diodes downregulate cathelicidin, kallikrein and toll-like receptor 2 expressions in keratinocytes and rosacea-like mouse skin.;Ginsenoside Rg5 Inhibits Succinate-Associated Lipolysis in Adipose Tissue and Prevents Muscle Insulin Resistance.;Effects of cold exposure on the bone marrow adipose tissue;Antimalarial activity of vitamin D3 (VD3) does not result from VD3-induced antimicrobial agents including nitric oxide or cathelicidin;Myeloid cell-derived LL-37 promotes lung cancer growth by activating Wnt/β-catenin signaling;