ELISA Kit for Hyaluronidase (HAase)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

78-5,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Mus musculus (Mouse)

Sensitivity

The minimum detectable dose of this kit is typically less than 35pg/mL

Sample type

serum, plasma, tissue homogenates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hyaluronidase (HAase). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hyaluronidase (HAase). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hyaluronidase (HAase), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hyaluronidase (HAase) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

Hyaluronic Lase; Hyaluroniclase

Item Name

Hyaluronidase

Research Area

Enzyme & Kinase;Tumor immunity;Hepatology;

Reference

Coordination Among Lipid Droplets, Peroxisomes and Mitochondria Regulates Energy Expenditure Through the CIDE-ATGL-PPARα Pathway in Adipocytes;6% Hydroxyethyl starch (HES 130/0.4) diminishes glycocalyx degradation and decreases vascular permeability during systemic and pulmonary inflammation …;Hyaluronan as a mediator for the hepatoprotective effect of Diosmin/Hesperidin complex.;