ELISA Kit for Somatostatin (SST)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

6.17-500pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 2.62pg/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Somatostatin (SST) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Somatostatin (SST) and unlabeled Somatostatin (SST) (Standards or samples) with the pre-coated antibody specific to Somatostatin (SST). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Somatostatin (SST) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Somatostatin (SST) in the sample.

Alternative Names

GHIH; SRIF; SMST; GHRIH; GHRIF; Growth Hormone Releasing Inhibiting Hormone; Somatotropin Release-Inhibiting Factor; Somatostatin-14; Somatostatin-28

Item Name

Somatostatin

Research Area

Tumor immunity;Endocrinology;Hormone metabolism;

Reference

Pancreatic Somatostatinoma Diagnosed Preoperatively: Report of a Case;Enhanced expression of neuropeptide S (NPS) receptor in eosinophils from severe asthmatics and subjects with total IgE above 100IU/ml;Colonic Transit Time and Gut Peptides in Adult Patients with Slow and Normal Colonic Transit Constipation;Somatostatin Maintains Permeability and Integrity of Blood-Brain Barrier in β-Amyloid Induced Toxicity;Home-Based Transcutaneous Neuromodulation Improved Constipation via Modulating Gastrointestinal Hormones and Bile Acids;