ELISA Kit for Follicle Stimulating Hormone (FSH)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

2.22-180ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Mus musculus (Mouse)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.84ng/mL

Sample type

serum, plasma and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Follicle Stimulating Hormone (FSH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Follicle Stimulating Hormone (FSH) and unlabeled Follicle Stimulating Hormone (FSH) (Standards or samples) with the pre-coated antibody specific to Follicle Stimulating Hormone (FSH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Follicle Stimulating Hormone (FSH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Follicle Stimulating Hormone (FSH) in the sample.

Alternative Names

Item Name

Follicle Stimulating Hormone

Research Area

Endocrinology;Reproductive science;Genetic science;Hormone metabolism;

Reference

FoxO1 Is a Negative Regulator of FSHβ Gene Expression in Basal and GnRH-Stimulated Conditions in Female;Targeted disruption of the mouse testis-enriched gene Znf230 does not affect spermatogenesis or fertility;New gonadotropin-releasing hormone glycolipids with direct antiproliferative activity and gonadotropin-releasing potency.;Pyrethroid Insecticide Cypermethrin Accelerates Pubertal Onset in Male Mice via Disrupting Hypothalamic-Pituitary-Gonadal Axis;Development of New Gonadotropin-Releasing Hormone-Modified Dendrimer Platforms with Direct Antiproliferative and Gonadotropin Releasing Activity;Pyrethroid Insecticide Cypermethrin Modulates Gonadotropin Synthesis via Calcium Homeostasis and ERK1/2 Signaling in LbT2 Mouse Pituitary Cells;Vitamin D deficiency impairs testicular development and spermatogenesis in mice;Lysophosphatidic acid acyltransferase 3 tunes the membrane status of germ cells by incorporating docosahexaenoic acid during spermatogenesis.;High sensitivity of testicular function to titanium nanoparticles;The Combination of icariin and constrained dynamic loading stimulation attenuates bone loss in ovariectomy‐induced osteoporotic mice;Effect of controlled ovarian hyperstimulation on puberty and estrus in mice offspring;Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island population;Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice;A Pex13 knockout in germ cells induces a spermatogenic arrest;Crocetin attenuates DHT-induced polycystic ovary syndrome in mice via revising kisspeptin neurons;Tanshinone IIA attenuates estradiol‑induced polycystic ovarian syndrome in mice by ameliorating FSHR expression in the ovary;Protective properties of glycogen synthase kinase-3 inhibition against doxorubicin-induced oxidative damage to mouse ovarian reserve;Menstrual blood derived mesenchymal stem cells combined with Bushen Tiaochong recipe improved chemotherapy-induced premature ovarian failure in …;