AAK1 Antibody

Size

100ug

Clone Number

Aliases

AAK 1 antibody; Aak1 antibody; AAK1_HUMAN antibody; Adaptor associated kinase 1 antibody; Adaptor-associated kinase 1 antibody; AP2 associated kinase 1 antibody; AP2 associated protein kinase 1 antibody; AP2-associated protein kinase 1 antibody; DKFZp686F03202 antibody; DKFZp686K16132 antibody; FLJ23712 antibody; FLJ25931 antibody; FLJ31060 antibody; FLJ42882 antibody; FLJ45252 antibody; KIAA1048 antibody; MGC138170 antibody; MGC164568 antibody; MGC164570 antibody

Immunogen Species

Homo sapiens (Human)

UniProt ID

Q2M2I8

Immunogen

Recombinant Human AP2-associated protein kinase 1 protein (274-465AA)

Raised in

Rabbit

Species Reactivity

Human, Mouse

Tested Applications

ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200

Background

Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity.

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Conjugate

Non-conjugated

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

AAK1

Research Areas

Signal transduction