6*His Monoclonal Antibody

Size

100ul

Clone Number

3G5H8

Aliases

6*His, 6*His, His Tag, 6histag, 6XHis, HHHHHH, HIS, His Tag

Immunogen Species

UniProt ID

Immunogen

HHHHHH(6x His) synthetic peptide conjugate to KLH

Raised in

Mouse

Species Reactivity

All

Tested Applications

ELISA, WB; Recommended dilution: WB:1:500-1:2000

Background

A His tag is a tag of 6 histidines added to the end of the protein that the DNA codes for, so the DNA with contain 6 repeats of the His codon. What it is used for is purifying the protein that the DNA codes for. Histidines are positively charged amino acids and a group of 6 of them allows the protein to bind to a negatively charged column. Histidines are also special in that they have a pKa around 7, meaning that once bound to the column they can easily be unbound.

Clonality

Monoclonal

Isotype

IgG2a

Purification Method

>95%, Protein A purified

Conjugate

Non-conjugated

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

6*His

Research Areas