{"product_id":"nsj-r31581-100ug","title":"TdT Antibody \/ DNA nucleotidylexotransferase, 100 ug","description":"\u003cp\u003eTerminal Deoxynucleotidyl Transferase, also known as Terminal transferase, is a unique DNA polymerase that without template direction catalyzes the addition of deoxyribonucleotides onto the 3-prime-hydroxyl end of DNA primers. Its gene is mapped to the region 10q23-q24. And TdT cDNA contains an open reading frame of 1,530 basepairs corresponding to a protein containing 510 amino acids. TdT may be responsible for inserting nucleotides (N regions) at the V(H)-D and D-J(H) junctions of immunoglobulin genes. The enzyme is present in immature thymocytes, some bone marrow cells, transformed pre-B and pre-T cell lines, and leukemia cells. Additionally, TdT catalyses the addition of nucleotides to the 3' terminus of a DNA molecule. Unlike most DNA polymerases it does not require a template. The preferred substrate of this enzyme is a 3'-overhang, but it can also add nucleotides to blunt or recessed 3' ends. Cobalt is a necessary cofactor.\u003c\/p\u003e","brand":"NSJ Bioreagents","offers":[{"title":"100 ug","offer_id":51550456709400,"sku":"R31581","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/get_image_344f27a1-173a-4ae4-aff5-9abd4cc28c91.jpg?v=1768314659","url":"https:\/\/danabiosci.com\/products\/nsj-r31581-100ug","provider":"Dana Bioscience","version":"1.0","type":"link"}