{"product_id":"nsj-fy13075-100ug","title":"PIK3C3 Antibody \/ VPS34 \/ Phosphatidylinositol 3-kinase catalytic subunit type 3, 100 ug","description":"\u003cp\u003ePIK3C3 antibody detects Phosphatidylinositol 3-kinase catalytic subunit type 3, a lipid kinase that generates phosphatidylinositol 3-phosphate (PI3P) to control autophagy and endosomal trafficking. The UniProt recommended name is Phosphatidylinositol 3-kinase catalytic subunit type 3 (PIK3C3), also known as Vacuolar protein sorting-associated protein 34 (VPS34). This enzyme is the only class III PI3K in mammalian cells and plays a pivotal role in autophagosome formation and lysosomal function.\u003cbr\u003e\u003cbr\u003eFunctionally, PIK3C3 antibody recognizes a 887-amino-acid cytoplasmic protein that catalyzes the phosphorylation of phosphatidylinositol to generate PI3P, recruiting downstream effectors such as WIPI and DFCP1. PIK3C3 forms multiprotein complexes with Beclin 1, UVRAG, ATG14, and VPS15 to regulate membrane nucleation during autophagy and endosome maturation. Its lipid kinase activity is crucial for endocytic trafficking, nutrient sensing, and organelle biogenesis.\u003cbr\u003e\u003cbr\u003eThe PIK3C3 gene, located on chromosome 18q12.3, is ubiquitously expressed and evolutionarily conserved from yeast to humans. It governs autophagic flux by mediating vesicle formation and fusion with lysosomes. Through its interaction with Beclin 1, PIK3C3 serves as a core regulator of cellular homeostasis under nutrient stress and is involved in tumor suppression and neuroprotection.\u003cbr\u003e\u003cbr\u003ePathologically, dysregulation of PIK3C3\/VPS34 contributes to cancer, neurodegeneration, and infectious disease. Inhibition of VPS34 impairs autophagy, leading to protein aggregation and metabolic imbalance. Conversely, enhanced PIK3C3 activity supports tumor cell survival under nutrient deprivation. The enzyme is also hijacked by viruses to generate replication compartments. Research using PIK3C3 antibody advances understanding of autophagic control, endosomal transport, and signaling adaptation to stress.\u003cbr\u003e\u003cbr\u003ePIK3C3 antibody is suitable for western blotting, immunofluorescence, and immunoprecipitation to detect VPS34 and its complexes. It supports studies in autophagy, intracellular trafficking, and lipid signaling. NSJ Bioreagents provides validated PIK3C3 antibody reagents optimized for autophagy and vesicle biogenesis research.\u003cbr\u003e\u003cbr\u003eStructurally, PIK3C3 consists of a helical domain, a C2 domain for membrane association, and a lipid kinase catalytic domain. Its activity depends on interactions with VPS15 and Beclin 1 for stabilization and subcellular localization. This antibody facilitates analysis of PIK3C3\/VPS34's central role in autophagy initiation and endolysosomal trafficking.\u003c\/p\u003e","brand":"NSJ Bioreagents","offers":[{"title":"100 ug","offer_id":51552335233304,"sku":"FY13075","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/get_image_75e90547-0d1b-4880-acf4-cff31d330304.jpg?v=1768353034","url":"https:\/\/danabiosci.com\/products\/nsj-fy13075-100ug","provider":"Dana Bioscience","version":"1.0","type":"link"}