{"product_id":"nsj-fy12730-100ug","title":"TPCN2 Antibody \/ Two-pore channel 2, 100 ug","description":"\u003cp\u003eTPCN2 antibody detects Two-pore channel 2, an endolysosomal cation channel that mediates calcium and sodium flux in response to nicotinic acid adenine dinucleotide phosphate (NAADP) signaling. Encoded by the TPCN2 gene on chromosome 11q13.4, this channel is part of the two-pore channel family that regulates intracellular calcium release from acidic organelles such as lysosomes and endosomes. TPCN2 contains two homologous pore-forming domains, each with six transmembrane helices, and functions as a homodimeric voltage-gated ion channel. It plays a key role in vesicle trafficking, autophagy, endosomal fusion, and cellular excitability.\u003cbr\u003e\u003cbr\u003eTPCN2 is widely expressed in kidney, liver, pancreas, and smooth muscle tissues, where it coordinates calcium-dependent signaling events. Activation of TPCN2 by NAADP triggers localized calcium release that modulates downstream pathways, including mTOR signaling, lysosomal exocytosis, and metabolic adaptation. Genetic studies have linked TPCN2 variants to pigmentation differences and hair color determination, highlighting its broader physiological influence. In neurons, TPCN2 regulates endolysosomal dynamics essential for synaptic vesicle recycling and signal transmission.\u003cbr\u003e\u003cbr\u003eThe TPCN2 antibody is a key reagent for cell signaling, membrane biology, and neurophysiology studies. Western blot analysis identifies a 75 kilodalton band corresponding to full-length TPCN2, while immunofluorescence reveals punctate staining consistent with lysosomal localization. The antibody enables detection of TPCN2 in subcellular fractionation and colocalization experiments with LAMP1 and Rab7 markers. Because endolysosomal calcium release impacts autophagy, metabolism, and exocytosis, this antibody supports research into pathways controlling intracellular trafficking and energy homeostasis.\u003cbr\u003e\u003cbr\u003eLoss or dysregulation of TPCN2 impairs lysosomal function, alters autophagic flux, and contributes to disorders involving calcium signaling imbalance. Elevated expression has been associated with cancer cell invasiveness and altered vesicular pH homeostasis. The TPCN2 antibody allows researchers to evaluate how NAADP-dependent calcium signaling influences physiological and pathological processes. NSJ Bioreagents provides this antibody validated for its applications, ensuring accurate and reproducible detection of Two-pore channel 2 across species.\u003c\/p\u003e","brand":"NSJ Bioreagents","offers":[{"title":"100 ug","offer_id":51552359022872,"sku":"FY12730","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/get_image_1fbcd75b-f199-4f09-b43a-4c6ff86aebaa.jpg?v=1768353612","url":"https:\/\/danabiosci.com\/products\/nsj-fy12730-100ug","provider":"Dana Bioscience","version":"1.0","type":"link"}