{"product_id":"nsj-cd163-antibody-primary-antibody","title":"Cd163 Antibody","description":"\u003cp\u003eCd163 is a type I transmembrane glycoprotein belonging to the scavenger receptor cysteine-rich (SRCR) superfamily. It is highly expressed on cells of the monocyte\/macrophage lineage, where it functions as a hemoglobin-haptoglobin complex receptor. By mediating clearance of hemoglobin from sites of tissue damage or intravascular hemolysis, Cd163 plays a central role in protecting tissues from oxidative stress and inflammation. A Cd163 antibody is frequently used to study macrophage biology, immune regulation, and tissue remodeling in both mouse and rat models.    \u0026lt;br\u0026gt;\u0026lt;br\u0026gt;  Cd163 expression is strongly associated with alternatively activated (M2) macrophages, which are involved in anti-inflammatory processes, tissue repair, and immunosuppression. This makes Cd163 a valuable marker for identifying macrophage polarization states. Employing a Cd163 antibody allows researchers to investigate the dynamics of macrophage activation in response to injury, infection, or disease progression.    \u0026lt;br\u0026gt;\u0026lt;br\u0026gt;  Beyond its role in hemoglobin clearance, Cd163 has broader immunomodulatory functions. Engagement of Cd163 can trigger anti-inflammatory signaling cascades and promote the release of heme oxygenase-1, further contributing to cytoprotection. Dysregulated Cd163 expression has been implicated in conditions such as sepsis, atherosclerosis, and chronic inflammatory diseases. In cancer, Cd163-positive macrophages are often linked to tumor-associated macrophages (TAMs), which can support tumor growth and immune evasion. A Cd163 antibody is therefore a powerful tool in both basic immunology and translational research.    \u0026lt;br\u0026gt;\u0026lt;br\u0026gt;  NSJ Bioreagents provides a high-quality Cd163 antibody validated for use in western blot, immunohistochemistry, and flow cytometry. Choosing a Cd163 antibody from NSJ Bioreagents ensures reliable performance and reproducibility in studies of macrophage biology, immune responses, and inflammation in rodent models.\u003c\/p\u003e\u003ch3\u003eSpecifications\u003c\/h3\u003e\u003ctable style=\"width:100%; border-collapse:collapse;\"\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eFamily\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003ePrimary antibody\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eFormulation\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003e0.5mg\/ml if reconstituted with 0.2ml sterile DI water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eFormat\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003eAntigen affinity purified\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eHost Animal\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003eRabbit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eClonality\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003ePolyclonal (rabbit origin)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eIsotype\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003eRabbit IgG\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eSpecies Reactivity\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003eMouse, Rat\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eApplication\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003eWB, IHC-P, FACS, ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eApplication Details\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003eWestern blot: 1-2ug\/ml,Immunohistochemistry (FFPE): 2-5ug\/ml,ELISA: 0.1-0.5ug\/ml,Flow cytometry: 1-3ug\/million cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eApplication Note\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003eOptimal dilution of the Cd163 antibody should be determined by the researcher.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eLocalization\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003eCell surface, cytoplasmic\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eImmunogen\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003eAmino acids N122-H1039 from the mouse protein were used as the immunogen for the Cd163 antibody.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eBuffer\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003eLyophilized from 1X PBS with 2% Trehalose\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003ePurity\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003eAntigen affinity purified\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eStorage\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003eAfter reconstitution, the Cd163 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eLimitation\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003eThis Cd163 antibody is available for research use only.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eUniprot #\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003eQ2VLH6\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eStatus\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003eAvailable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003ePDF Link\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003e\u003ca href=\"http:\/\/www.nsjbio.com\/tds-pdf\/cd163-antibody-rq8935\" target=\"_blank\" rel=\"noopener noreferrer\"\u003ehttp:\/\/www.nsjbio.com\/tds-pdf\/cd163-antibody-rq8935\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eTitle\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003eCd163 Antibody\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eDescription\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003eCd163 is a type I transmembrane glycoprotein belonging to the scavenger receptor cysteine-rich (SRCR) superfamily. It is highly expressed on cells of the monocyte\/macrophage lineage, where it functions as a hemoglobin-haptoglobin complex receptor. By mediating clearance of hemoglobin from sites of tissue damage or intravascular hemolysis, Cd163 plays a central role in protecting tissues from oxidative stress and inflammation. A Cd163 antibody is frequently used to study macrophage biology, immune regulation, and tissue remodeling in both mouse and rat models.    \u0026lt;br\u0026gt;\u0026lt;br\u0026gt;  Cd163 expression is strongly associated with alternatively activated (M2) macrophages, which are involved in anti-inflammatory processes, tissue repair, and immunosuppression. This makes Cd163 a valuable marker for identifying macrophage polarization states. Employing a Cd163 antibody allows researchers to investigate the dynamics of macrophage activation in response to injury, infection, or disease progression.    \u0026lt;br\u0026gt;\u0026lt;br\u0026gt;  Beyond its role in hemoglobin clearance, Cd163 has broader immunomodulatory functions. Engagement of Cd163 can trigger anti-inflammatory signaling cascades and promote the release of heme oxygenase-1, further contributing to cytoprotection. Dysregulated Cd163 expression has been implicated in conditions such as sepsis, atherosclerosis, and chronic inflammatory diseases. In cancer, Cd163-positive macrophages are often linked to tumor-associated macrophages (TAMs), which can support tumor growth and immune evasion. A Cd163 antibody is therefore a powerful tool in both basic immunology and translational research.    \u0026lt;br\u0026gt;\u0026lt;br\u0026gt;  NSJ Bioreagents provides a high-quality Cd163 antibody validated for use in western blot, immunohistochemistry, and flow cytometry. Choosing a Cd163 antibody from NSJ Bioreagents ensures reliable performance and reproducibility in studies of macrophage biology, immune responses, and inflammation in rodent models.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e","brand":"NSJ Bioreagents","offers":[{"title":"100 ug","offer_id":51544612208920,"sku":"RQ8935","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/get_image_5535a2f2-53c1-4302-b86f-4e05ae89f603.jpg?v=1768178025","url":"https:\/\/danabiosci.com\/products\/nsj-cd163-antibody-primary-antibody","provider":"Dana Bioscience","version":"1.0","type":"link"}