{"product_id":"nsj-axin1-antibody-primary-antibody","title":"AXIN1 Antibody","description":"\u003cp\u003eAXIN1 is a component of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling. Controls dorsoventral patterning via two opposing effects; down-regulates CTNNB1 to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway. In Wnt signaling, probably facilitates the phosphorylation of CTNNB1 and APC by GSK3B. Likely to function as a tumor suppressor. Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation. Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7. Also component of the AXIN1-HIPK2-TP53 complex which controls cell growth, apoptosis and development. [UniProt]\u003c\/p\u003e\u003ch3\u003eSpecifications\u003c\/h3\u003e\u003ctable style=\"width:100%; border-collapse:collapse;\"\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eFamily\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003ePrimary antibody\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eFormulation\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003eIn 1X PBS, pH 7.4, with 0.09% sodium azide\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eFormat\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003eAntigen affinity purified\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eHost Animal\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003eRabbit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eClonality\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003ePolyclonal (rabbit origin)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eIsotype\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003eRabbit Ig\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eSpecies Reactivity\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003ePredicted Species Reactivity\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003eMouse, Rat\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eApplication\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003eWB, ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eApplication Details\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003eWestern blot: 1:1000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eApplication Note\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003eTitration of the AXIN1 antibody may be required due to differences in protocols and secondary\/substrate sensitivity.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eImmunogen\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003eA portion of amino acids 330-359 from the human protein was used as the immunogen for this AXIN1 antibody.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003ePurity\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003eAntigen affinity\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eStorage\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003eAliquot the AXIN1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eLimitation\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003eThis AXIN1 antibody is available for research use only.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eUniprot #\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003eO15169\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eStatus\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003eAvailable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003ePDF Link\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003e\u003ca href=\"https:\/\/www.nsjbio.com\/tds-pdf\/axin1-antibody-f45423\" target=\"_blank\" rel=\"noopener noreferrer\"\u003ehttps:\/\/www.nsjbio.com\/tds-pdf\/axin1-antibody-f45423\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eTitle\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003eAXIN1 Antibody\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eDescription\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003eAXIN1 is a component of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling. Controls dorsoventral patterning via two opposing effects; down-regulates CTNNB1 to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway. In Wnt signaling, probably facilitates the phosphorylation of CTNNB1 and APC by GSK3B. Likely to function as a tumor suppressor. Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation. Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7. Also component of the AXIN1-HIPK2-TP53 complex which controls cell growth, apoptosis and development. [UniProt]\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.08 ml","offer_id":51543761813784,"sku":"F45423-0.08ML","price":205.0,"currency_code":"USD","in_stock":true},{"title":"0.4 ml","offer_id":51543761846552,"sku":"F45423-0.4ML","price":439.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":51543761879320,"sku":"R34056-100UG","price":475.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/get_image_43f8a0d5-aac3-46b3-bb6f-8c934bcceb2e.jpg?v=1768136136","url":"https:\/\/danabiosci.com\/products\/nsj-axin1-antibody-primary-antibody","provider":"Dana Bioscience","version":"1.0","type":"link"}