{"product_id":"nsj-acta2-antibody-alpha-actin-smooth-muscle-actin-primary-antibody","title":"ACTA2 Antibody \/ Alpha Actin \/ Smooth Muscle Actin","description":"\u003cp\u003eACTA2 antibody is a widely used reagent for studying cytoskeletal organization, smooth muscle biology, and tissue remodeling. The encoded protein, alpha actin (ACTA2), is an isoform of actin expressed primarily in vascular and visceral smooth muscle. As a major component of the contractile apparatus, ACTA2 plays a central role in generating force for smooth muscle contraction and maintaining structural integrity of the cytoskeleton.\u0026lt;br\u0026gt;\u0026lt;br\u0026gt;Alpha actin is abundantly expressed in smooth muscle cells of the vasculature, airways, gastrointestinal tract, and reproductive system. It is also used as a marker of myofibroblast differentiation, where its upregulation reflects tissue remodeling during wound healing, fibrosis, and pathological scarring. Because of its strong and specific expression pattern, detection with ACTA2 antibody is widely applied in immunohistochemistry and immunofluorescence to identify smooth muscle or myofibroblastic cells in tissue samples.\u0026lt;br\u0026gt;\u0026lt;br\u0026gt;In cardiovascular research, ACTA2 is studied as a marker of vascular smooth muscle cells and their phenotypic switching. Under pathological conditions such as atherosclerosis or hypertension, ACTA2 expression can change as smooth muscle cells adopt synthetic phenotypes. In pulmonary and hepatic fibrosis, ACTA2 positive myofibroblasts contribute to extracellular matrix deposition and tissue stiffening, making ACTA2 a reliable indicator of fibrotic remodeling.\u0026lt;br\u0026gt;\u0026lt;br\u0026gt;Structurally, alpha actin is a globular protein that polymerizes into filamentous actin, forming part of the cytoskeleton. ACTA2 interacts with actin binding proteins to regulate filament dynamics, cell shape, and motility. Its high conservation across species ensures that ACTA2 antibody is effective in many experimental systems, including human and animal models.\u0026lt;br\u0026gt;\u0026lt;br\u0026gt;The ACTA2 antibody is commonly used in western blotting, immunohistochemistry, immunofluorescence, and flow cytometry. These applications enable researchers to assess expression, distribution, and structural changes of smooth muscle actin in both healthy and diseased tissues. For scientists investigating vascular biology, fibrosis, tissue remodeling, or smooth muscle physiology, the ACTA2 antibody provides a sensitive and specific detection tool. NSJ Bioreagents offers validated antibodies that ensure reproducibility and accuracy in advanced molecular studies.\u003c\/p\u003e\u003ch3\u003eSpecifications\u003c\/h3\u003e\u003ctable style=\"width:100%; border-collapse:collapse;\"\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eFamily\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003ePrimary antibody\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eFormulation\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003eAntibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg\/ml BSA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eFormat\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003ePurified\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eClone\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003eEO-1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eHost Animal\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003eRabbit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eClonality\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003eRecombinant Rabbit Monoclonal\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eIsotype\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003eRabbit IgG\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eSpecies Reactivity\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003eHuman, Mouse, Rat\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eApplication\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003eWB, IHC-P, IF\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eApplication Details\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003eWestern blot: 1:500,Immunohistochemistry (FFPE): 1:50,Immunofluorescence: 1:50\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eApplication Note\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003eOptimal dilution of the ACTA2 antibody should be determined by the researcher.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eLocalization\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003eCytoplasmic\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eImmunogen\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003eA synthetic peptide specific to human Smooth muscle actin was used as the immunogen for the ACTA2 antibody.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003ePurity\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003eAffinity purified\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eStorage\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003eStore the ACTA2 antibody at -20oC.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eLimitation\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003eThis ACTA2 antibody is available for research use only.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eUniprot #\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003eP62736\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eStatus\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003eAvailable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003ePDF Link\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003e\u003ca href=\"http:\/\/www.nsjbio.com\/tds-pdf\/acta2-antibody-alpha-actin-smooth-muscle-actin-rq8952\" target=\"_blank\" rel=\"noopener noreferrer\"\u003ehttp:\/\/www.nsjbio.com\/tds-pdf\/acta2-antibody-alpha-actin-smooth-muscle-actin-rq8952\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eTitle\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003eACTA2 Antibody \/ Alpha Actin \/ Smooth Muscle Actin\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left; vertical-align:top; padding:6px; border-bottom:1px solid #eee;\"\u003eDescription\u003c\/th\u003e\n\u003ctd style=\"padding:6px; border-bottom:1px solid #eee;\"\u003eACTA2 antibody is a widely used reagent for studying cytoskeletal organization, smooth muscle biology, and tissue remodeling. The encoded protein, alpha actin (ACTA2), is an isoform of actin expressed primarily in vascular and visceral smooth muscle. As a major component of the contractile apparatus, ACTA2 plays a central role in generating force for smooth muscle contraction and maintaining structural integrity of the cytoskeleton.\u0026lt;br\u0026gt;\u0026lt;br\u0026gt;Alpha actin is abundantly expressed in smooth muscle cells of the vasculature, airways, gastrointestinal tract, and reproductive system. It is also used as a marker of myofibroblast differentiation, where its upregulation reflects tissue remodeling during wound healing, fibrosis, and pathological scarring. Because of its strong and specific expression pattern, detection with ACTA2 antibody is widely applied in immunohistochemistry and immunofluorescence to identify smooth muscle or myofibroblastic cells in tissue samples.\u0026lt;br\u0026gt;\u0026lt;br\u0026gt;In cardiovascular research, ACTA2 is studied as a marker of vascular smooth muscle cells and their phenotypic switching. Under pathological conditions such as atherosclerosis or hypertension, ACTA2 expression can change as smooth muscle cells adopt synthetic phenotypes. In pulmonary and hepatic fibrosis, ACTA2 positive myofibroblasts contribute to extracellular matrix deposition and tissue stiffening, making ACTA2 a reliable indicator of fibrotic remodeling.\u0026lt;br\u0026gt;\u0026lt;br\u0026gt;Structurally, alpha actin is a globular protein that polymerizes into filamentous actin, forming part of the cytoskeleton. ACTA2 interacts with actin binding proteins to regulate filament dynamics, cell shape, and motility. Its high conservation across species ensures that ACTA2 antibody is effective in many experimental systems, including human and animal models.\u0026lt;br\u0026gt;\u0026lt;br\u0026gt;The ACTA2 antibody is commonly used in western blotting, immunohistochemistry, immunofluorescence, and flow cytometry. These applications enable researchers to assess expression, distribution, and structural changes of smooth muscle actin in both healthy and diseased tissues. For scientists investigating vascular biology, fibrosis, tissue remodeling, or smooth muscle physiology, the ACTA2 antibody provides a sensitive and specific detection tool. NSJ Bioreagents offers validated antibodies that ensure reproducibility and accuracy in advanced molecular studies.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e","brand":"NSJ Bioreagents","offers":[{"title":"100 ul","offer_id":51543742251288,"sku":"RQ8952","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/get_image_0ec6c4b7-6a23-47e2-b97d-0eac37a4bb37.jpg?v=1768135192","url":"https:\/\/danabiosci.com\/products\/nsj-acta2-antibody-alpha-actin-smooth-muscle-actin-primary-antibody","provider":"Dana Bioscience","version":"1.0","type":"link"}