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SKU:PAB301Ge01

Polyclonal Antibody to Asymmetrical Dimethylarginine (ADMA)

Polyclonal Antibody to Asymmetrical Dimethylarginine (ADMA)

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UOM
Concentration
Organism species

Immunohistochemistry: 5-20µg/mL;
;Immunocytochemistry: 5-20µg/mL;
;Optimal working dilutions must be determined by end user.

Product No.

PAB301Ge01

Organism Species

Pan-species (General).

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

CPB301Ge11-BSA Conjugated Asymmetrical Dimethylarginine (ADMA)

Buffer Formulation

0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Traits

Liquid

Concentration

0.45mg/ml

Organism Species More

Pan-species (General)

Applications

ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against ADMA. It has been selected for its ability to recognize ADMA in immunohistochemical staining and western blotting.

USAGE

Enzyme-Linked Immune Sorbent Assay: 200ng/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine

Citations

PLoS One.

Interactions among Vascular-Tone Modulators Contribute to High Altitude Pulmonary Edema and Augmented Vasoreactivity in Highlanders PubMed: PMC3439466

Cytokine

Effect of CCL5 on dimethylarginine dimethylaminohydrolase-1 production in vascular smooth muscle cells from spontaneously hypertensive rats. Pubmed: 23834755

Neurol Res.

Effect of 18β-glycyrrhetinic acid on cerebral vasospasm caused by asymmetric dimethylarginine after experimental subarachnoid hemorrhage in rats. Pubmed:25475507

Int J Clin Exp Med.

Correlation research on ADMA plasma levels and left ventricular function of peritoneal dialysis patients Pubmed:25550968

Clin Respir J

Asymmetric dimethylarginine contributes to airway nitric oxide deficiency in patients with COPD PubMed: 26076870

Cytokine

Dimethylarginine dimethylaminohydrolase-1 mediates inhibitory effect of interleukin-10 on angiotensin II-induced hypertensive effects in vascular smooth muscle cells of spontaneously hypertensive rats PubMed: 26375520

Neurological Research

Effect of 18β-glycyrrhetinic acid on cerebral vasospasm caused by asymmetric dimethylarginine after experimental subarachnoid hemorrhage in rats PubMed: 25475507

Scientific Reports

Dimethylarginine Dimethylaminohydrolase 2 (DDAH 2) Gene Polymorphism, Asymmetric Dimethylarginine (ADMA) Concentrations, and Risk of Coronary Artery Disease: A Case-Control Study pubmed:27677852

clinica chimica acta

Increased serum concentrations of asymmetric dimethylarginine (ADMA) in patients with early-onset coronary artery disease. pubmed:27884754

The Journal of Physiology

Asymmetric dimethylarginine (ADMA) elevation and arginase up-regulation contribute toendothelial dysfunction related to insulin resistance in rats and morbidly obese humans. pubmed:26840628

Angiogenesis

Dimethylarginine dimethylaminohydrolase-1 (DDAH1) is frequently upregulated in prostate cancer, and its overexpression conveys tumor growth and angiogenesis by metabolizing asymmetric dimethylarginine (ADMA). pubmed:29150732

Scientific Reports

A Novel Role of Irbesartan in Gastroprotection against Indomethacin-Induced Gastric Injury in Rats: Targeting DDAH/ADMA and EGFR/ERK Signaling Pubmed:29523851

Mechanisms of Ageing and Development

Multivessel analysis of progressive vascular aging in the rat: Asynchronous vulnerability among vascular territories Pubmed:29605639

Nutrition, Metabolism and Cardiovascular Diseases

Arginine bioavailability and endothelin-1 system in the regulation of vascular function of umbilical vein endothelial cells from intrauterine growth restricted newborns Doi: 10.1016/j.numecd.2018.09.002

Biochemical and Biophysical Research Communications

Probucol improves erectile function via Activation of Nrf2 and coordinates the HO-1/DDAH/PPAR-γ/eNOS pathways in streptozotocin-induced diabetic rats Pubmed: 30454888

Brazilian Journal of Pharmaceutical Science

Demographic, clinical and lifestyle predictors for severity of erectile dysfunction and biomarkers level in Malaysian patients

ACS Combinatorial Science

Novel cellularly active inhibitor regresses DDAH1 induced prostate tumor growth by restraining tumor angiogenesis through targeting DDAH1/ADMA/NOS pathway Pubmed: 30673277

International Journal of the Cardiovascular Academy

Nebivolol prevents the increase of asymmetric dimethylarginine and oxidants in hyperhomocysteinemic rats

Oxid Med Cell Longev

The Role of Reactive Oxygen Species and Nitric Oxide in the Inhibition of Trichophyton rubrum Growth by HaCaT Cells Pubmed: 32104540

Molecular Medicine Reports

Blueberry anthocyanin‑enriched extract ameliorates transverse aortic constriction‑induced myocardial dysfunction via the DDAH1/ADMA/NO signaling pathway in … Pubmed: 31746378

International Journal of Nephrology

Effect of a Supervised Peridialytic Exercise Program on Serum Asymmetric Dimethylarginine in Maintenance Hemodialysis Patients Pubmed: 33163233

Journal of Clinical Medicine

The Usefulness of Serum Biomarkers in the Early Stages of Diabetic Retinopathy: Results of the EUROCONDOR Clinical Trial Pubmed: 32344735

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

Levels of endothelial substances in patients with newly identified hypertension compared with healthy controls 33463628

WIENER KLINISCHE WOCHENSCHRIFT

Non-functioning adrenal incidentalomas may increase toxic metabolites 34255169

pharmaceuticals

Effect of a Low Dose of Carvedilol on Cyclophosphamide-Induced Urinary Toxicity in Rats—A Comparison with Mesna 34959638

Int J Biochem Cell Biol

HIF-1α and Nrf2 regulates hypoxia induced overexpression of DDAH1 through promoter activation in prostate cancer Pubmed:35644470


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