{"product_id":"new-product-643570","title":"Monoclonal Antibody to Corticotropin Releasing Factor (CRF)","description":"\u003cp\u003eWestern blotting: 0.5-2µg\/mL\u003cbr\u003e;Immunohistochemistry: 5-20µg\/mL\u003cbr\u003e;Immunocytochemistry: 5-20µg\/mL\u003cbr\u003e;Optimal working dilutions must be determined by end user.\u003c\/p\u003e\n\u003cp\u003eProduct No.\u003c\/p\u003e\n\u003cp\u003eMAA835Mi21\u003c\/p\u003e\n\u003cp\u003eOrganism Species\u003c\/p\u003e\n\u003cp\u003eHomo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Canis familiaris; Canine (Dog), Sus scrofa; Porcine (Pig).\u003c\/p\u003e\n\u003cp\u003eSource\u003c\/p\u003e\n\u003cp\u003eMonoclonal antibody preparation\u003c\/p\u003e\n\u003cp\u003eHost\u003c\/p\u003e\n\u003cp\u003eMouse\u003c\/p\u003e\n\u003cp\u003ePotency (Clone Number)\u003c\/p\u003e\n\u003cp\u003e1#\u003c\/p\u003e\n\u003cp\u003eIg Isotype\u003c\/p\u003e\n\u003cp\u003eIgG2a Kappa\u003c\/p\u003e\n\u003cp\u003ePurification\u003c\/p\u003e\n\u003cp\u003eProtein A + Protein G affinity chromatography\u003c\/p\u003e\n\u003cp\u003eLabel\u003c\/p\u003e\n\u003cp\u003eNone\u003c\/p\u003e\n\u003cp\u003eImmunogen\u003c\/p\u003e\n\u003cp\u003en\/a\u003c\/p\u003e\n\u003cp\u003eBuffer Formulation\u003c\/p\u003e\n\u003cp\u003e0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.\u003c\/p\u003e\n\u003cp\u003eTraits\u003c\/p\u003e\n\u003cp\u003eLiquid\u003c\/p\u003e\n\u003cp\u003eConcentration\u003c\/p\u003e\n\u003cp\u003e0.8mg\/ml\u003c\/p\u003e\n\u003cp\u003eOrganism Species More\u003c\/p\u003e\n\u003cp\u003eHomo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Canis familiaris; Canine (Dog), Sus scrofa; Porcine (Pig)\u003c\/p\u003e\n\u003cp\u003eApplications\u003c\/p\u003e\n\u003cp\u003eWB; IHC; ICC; IP.\u003c\/p\u003e\n\u003cp\u003eIf the antibody is used in flow cytometry, please check FCM antibodies.\u003c\/p\u003e\n\u003cp\u003eUOM\u003c\/p\u003e\n\u003cp\u003e20µl 100µl 200µl 1ml 10ml\u003c\/p\u003e\n\u003ch3\u003eSPECIFITY\u003c\/h3\u003e\n\u003cdiv\u003e\n\u003cp\u003eThe antibody is a mouse monoclonal antibody raised against CRF. It has been selected for its ability to recognize CRF in immunohistochemical staining and western blotting.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch3\u003eUSAGE\u003c\/h3\u003e\n\u003cdiv class=\"img_parent\"\u003e\n\u003cp\u003eWestern blotting: 0.5-2µg\/mL\u003cbr\u003eImmunohistochemistry: 5-20µg\/mL\u003cbr\u003eImmunocytochemistry: 5-20µg\/mL\u003cbr\u003eOptimal working dilutions must be determined by end user.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch3\u003eSTORAGE\u003c\/h3\u003e\n\u003cdiv\u003e\n\u003cp\u003eStore at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch3\u003eSTABILITY\u003c\/h3\u003e\n\u003cdiv\u003e\n\u003cp\u003eThe thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.\u003c\/p\u003e\n\u003cdiv class=\"product_reference\"\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr class=\"first_tr\"\u003e\n\u003ctd class=\"td_1\"\u003eMagazine\u003c\/td\u003e\n\u003ctd\u003eCitations\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eToxicology Letters\u003c\/td\u003e\n\u003ctd\u003eRegulation of corticosterone secretion is modified by PFOS exposure at different levels of the hypothalamic–pituitary–adrenal axis in adult male rats\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/24440345\" target=\"_blank\"\u003ePubmed:24440345\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBipolar?Disord.\u003c\/td\u003e\n\u003ctd\u003eImmune dysfunction in bipolar disorder and suicide risk: is there an association between peripheral corticotropin‐releasing hormone and interleukin‐1β?\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/24862833\" target=\"_blank\"\u003ePubmed:24862833\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePeptides\u003c\/td\u003e\n\u003ctd\u003eDifferential stress response in rats subjected to chronic mild stress is accompanied by changes in CRH-family gene expression at the pituitary level\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/25236411\" target=\"_blank\"\u003ePubmed:25236411\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAnn Dermatol\u003c\/td\u003e\n\u003ctd\u003eAcute Stress-Induced Changes in Follicular Dermal Papilla Cells and Mobilization of Mast Cells:Implications for Hair Growth.\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/27746640\" target=\"_blank\"\u003epubmed:27746640\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eNeuroscience\u003c\/td\u003e\n\u003ctd\u003eHeat stress-induced neuroinflammation and aberration in monoamine levels in hypothalamus are associated with temperature dysregulation\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/28663093\" target=\"_blank\"\u003epubmed:28663093\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiomed Research International\u003c\/td\u003e\n\u003ctd\u003eColonic Transit Time and Gut Peptides in Adult Patients with Slow and Normal Colonic Transit Constipation\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29082242\" target=\"_blank\"\u003epubmed:29082242\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiochemical Pharmacology\u003c\/td\u003e\n\u003ctd\u003eEffects of n-3 PUFA enriched and n-3 PUFA deficient diets in naïve and Aβ-treated female rats\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/30028991\" target=\"_blank\"\u003ePubmed:30028991\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFrontiers in Pharmacology\u003c\/td\u003e\n\u003ctd\u003eVaccinium bracteatum Leaf Extract Reverses Chronic Restraint Stress-Induced Depression-Like Behavior in Mice: Regulation of Hypothalamic-Pituitary …\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/30038568\" target=\"_blank\"\u003ePubmed:30038568\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eToxicology Letters\u003c\/td\u003e\n\u003ctd\u003ePerfluorooctane sulfonate (PFOS) can alter the hypothalamic-pituitary-adrenal (HPA) axis activity by modifying CRF1 and glucocorticoid receptors\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29807116\" target=\"_blank\"\u003ePubmed:29807116\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eNeuropsychiatric disease and treatment\u003c\/td\u003e\n\u003ctd\u003eThe depressive-like behaviors of chronic unpredictable mild stress-treated mice, ameliorated by Tibetan medicine Zuotai: involvement in the hypothalamic …\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29379286\" target=\"_blank\"\u003ePubmed:29379286\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBMC Veterinary Research\u003c\/td\u003e\n\u003ctd\u003eHypothalamus-pituitary-adrenal axis involves in anti-viral ability through regulation of immune response in piglets infected by highly pathogenic porcine …\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29540178\" target=\"_blank\"\u003ePubmed:29540178\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBioscience Research\u003c\/td\u003e\n\u003ctd\u003eBioscience Research\u003ca rel=\"nofollow\" href=\"https:\/\/www.isisn.org\/BR15(4)2018\/3472-3479-15(4)2018BR18-414.pdf\" target=\"_blank\"\u003e\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFASEB Journal\u003c\/td\u003e\n\u003ctd\u003eContribution of DNA methylation in chronic stress–induced cardiac remodeling and arrhythmias in mice\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"https:\/\/www.fasebj.org\/doi\/abs\/10.1096\/fj.201900100R\" target=\"_blank\"\u003ePubmed: 31431066\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAging Cell\u003c\/td\u003e\n\u003ctd\u003eAltered glucocorticoid metabolism represents a feature of macroph‐aging\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32463582\/\" target=\"_blank\"\u003ePubmed: 32463582\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEndocr J\u003c\/td\u003e\n\u003ctd\u003eImpairment of CRH in the intestinal mucosal epithelial barrier of pregnant Bama miniature pig induced by restraint stress\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/33408312\" target=\"_blank\"\u003e33408312\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCRYOBIOLOGY\u003c\/td\u003e\n\u003ctd\u003eO-GlcNAc\/Akt pathway regulates glucose metabolism and reduces apoptosis in liver of piglets with acute cold stress\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/33651993\" target=\"_blank\"\u003e33651993\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"product_relation\"\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr class=\"first_tr\"\u003e\n\u003ctd class=\"td_1\"\u003eCatalog No.\u003c\/td\u003e\n\u003ctd class=\"td_2\"\u003eRelated products for research use of Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Canis familiaris; Canine (Dog), Sus scrofa; Porcine (Pig) Organism species\u003c\/td\u003e\n\u003ctd class=\"td_3\"\u003eApplications (RESEARCH USE ONLY!)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMAA835Mi21\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/MAA835Mi21.html\"\u003eMonoclonal Antibody to Corticotropin Releasing Factor (CRF)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eWB; IHC; ICC; IP.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Cloud-clone","offers":[{"title":"200µl \/ 1mg\/mL \/ Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Canis familiaris; Canine (Dog), Sus scrofa; Porcine (Pig)","offer_id":47594284089624,"sku":"MAA835Mi21","price":366.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/Logo_Cloud_Clone_38014717-32b1-459d-b3d2-318d11153ed8.png?v=1706291384","url":"https:\/\/danabiosci.com\/products\/new-product-643570","provider":"Dana Bioscience","version":"1.0","type":"link"}