{"product_id":"new-product-642016","title":"ELISA Kit for Synuclein Alpha (SNCa)","description":"\u003cp\u003eEnzyme-linked immunosorbent assay for Antigen Detection.\u003c\/p\u003e\n\u003cp\u003eProduct No.\u003c\/p\u003e\n\u003cp\u003eSEB222Hu\u003c\/p\u003e\n\u003cp\u003eOrganism Species\u003c\/p\u003e\n\u003cp\u003eHomo sapiens (Human).\u003c\/p\u003e\n\u003cp\u003eSample Type\u003c\/p\u003e\n\u003cp\u003eserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003c\/p\u003e\n\u003cp\u003eTest Method\u003c\/p\u003e\n\u003cp\u003eDouble-antibody Sandwich\u003c\/p\u003e\n\u003cp\u003eAssay Length\u003c\/p\u003e\n\u003cp\u003e3h\u003c\/p\u003e\n\u003cp\u003eDetection Range\u003c\/p\u003e\n\u003cp\u003e0.156-10ng\/mL\u003c\/p\u003e\n\u003cp\u003eSensitivity\u003c\/p\u003e\n\u003cp\u003eThe minimum detectable dose of this kit is typically less than 0.065ng\/mL.\u003c\/p\u003e\n\u003cp\u003eUOM\u003c\/p\u003e\n\u003cp\u003e48T 96T 96T*5 96T*10 96T*100\u003c\/p\u003e\n\u003ch3\u003eSpecificity\u003c\/h3\u003e\n\u003cdiv\u003e\n\u003cp\u003eThis assay has high sensitivity and excellent specificity for detection of Synuclein Alpha (SNCa).\u003cbr\u003eNo significant cross-reactivity or interference between Synuclein Alpha (SNCa) and analogues was observed.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch3\u003eRecovery\u003c\/h3\u003e\n\u003cdiv\u003e\n\u003cp\u003eMatrices listed below were spiked with certain level of recombinant Synuclein Alpha (SNCa) and the recovery rates were calculated by comparing the measured value to the expected amount of Synuclein Alpha (SNCa) in samples.\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eMatrix\u003c\/td\u003e\n\u003ctd\u003eRecovery range (%)\u003c\/td\u003e\n\u003ctd\u003eAverage(%)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eserum(n=5)\u003c\/td\u003e\n\u003ctd\u003e86-97\u003c\/td\u003e\n\u003ctd\u003e93\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEDTA plasma(n=5)\u003c\/td\u003e\n\u003ctd\u003e88-96\u003c\/td\u003e\n\u003ctd\u003e92\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eheparin plasma(n=5)\u003c\/td\u003e\n\u003ctd\u003e87-94\u003c\/td\u003e\n\u003ctd\u003e91\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3\u003ePrecision\u003c\/h3\u003e\n\u003cdiv\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Synuclein Alpha (SNCa) were tested 20 times on one plate, respectively.\u003cbr\u003eInter-assay Precision (Precision between assays): 3 samples with low, middle and high level Synuclein Alpha (SNCa) were tested on 3 different plates, 8 replicates in each plate.\u003cbr\u003eCV(%) = SD\/meanX100\u003cbr\u003eIntra-Assay: CV\u0026lt;10%\u003cbr\u003eInter-Assay: CV\u0026lt;12%\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch3\u003eLinearity\u003c\/h3\u003e\n\u003cdiv\u003e\n\u003cp\u003eThe linearity of the kit was assayed by testing samples spiked with appropriate concentration of Synuclein Alpha (SNCa) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample\u003c\/td\u003e\n\u003ctd\u003e1:2\u003c\/td\u003e\n\u003ctd\u003e1:4\u003c\/td\u003e\n\u003ctd\u003e1:8\u003c\/td\u003e\n\u003ctd\u003e1:16\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eserum(n=5)\u003c\/td\u003e\n\u003ctd\u003e89-101%\u003c\/td\u003e\n\u003ctd\u003e78-101%\u003c\/td\u003e\n\u003ctd\u003e93-101%\u003c\/td\u003e\n\u003ctd\u003e87-94%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEDTA plasma(n=5)\u003c\/td\u003e\n\u003ctd\u003e92-103%\u003c\/td\u003e\n\u003ctd\u003e80-96%\u003c\/td\u003e\n\u003ctd\u003e89-101%\u003c\/td\u003e\n\u003ctd\u003e86-98%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eheparin plasma(n=5)\u003c\/td\u003e\n\u003ctd\u003e97-104%\u003c\/td\u003e\n\u003ctd\u003e84-103%\u003c\/td\u003e\n\u003ctd\u003e84-93%\u003c\/td\u003e\n\u003ctd\u003e80-96%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3\u003eStability\u003c\/h3\u003e\n\u003cdiv\u003e\n\u003cp\u003eThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.\u003cbr\u003eTo minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch3\u003eReagents and materials provided\u003c\/h3\u003e\n\u003cdiv\u003e\n\u003cdiv class=\"reagent_div\"\u003e\n\u003ctable class=\"reagent_table\"\u003e\n\u003ctbody\u003e\n\u003ctr class=\"first_tr\"\u003e\n\u003ctd\u003eReagents\u003c\/td\u003e\n\u003ctd\u003eQuantity\u003c\/td\u003e\n\u003ctd\u003eReagents\u003c\/td\u003e\n\u003ctd\u003eQuantity\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePre-coated, ready to use 96-well strip plate\u003c\/td\u003e\n\u003ctd\u003e1\u003c\/td\u003e\n\u003ctd\u003ePlate sealer for 96 wells\u003c\/td\u003e\n\u003ctd\u003e4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard\u003c\/td\u003e\n\u003ctd\u003e2\u003c\/td\u003e\n\u003ctd\u003eStandard Diluent\u003c\/td\u003e\n\u003ctd\u003e1×20mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDetection Reagent A\u003c\/td\u003e\n\u003ctd\u003e1×120µL\u003c\/td\u003e\n\u003ctd\u003eAssay Diluent A\u003c\/td\u003e\n\u003ctd\u003e1×12mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDetection Reagent B\u003c\/td\u003e\n\u003ctd\u003e1×120µL\u003c\/td\u003e\n\u003ctd\u003eAssay Diluent B\u003c\/td\u003e\n\u003ctd\u003e1×12mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTMB Substrate\u003c\/td\u003e\n\u003ctd\u003e1×9mL\u003c\/td\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd\u003e1×6mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer (30 × concentrate)\u003c\/td\u003e\n\u003ctd\u003e1×20mL\u003c\/td\u003e\n\u003ctd\u003eInstruction manual\u003c\/td\u003e\n\u003ctd\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch3\u003eAssay procedure summary\u003c\/h3\u003e\n\u003cdiv\u003e\n\u003cp\u003e1. Prepare all reagents, samples and standards;\u003cbr\u003e2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;\u003cbr\u003e3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;\u003cbr\u003e4. Aspirate and wash 3 times;\u003cbr\u003e5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;\u003cbr\u003e6. Aspirate and wash 5 times;\u003cbr\u003e7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;\u003cbr\u003e8. Add 50µL Stop Solution. Read at 450nm immediately.\u003c\/p\u003e\n\u003cdiv class=\"product_reference\"\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr class=\"first_tr\"\u003e\n\u003ctd class=\"td_1\"\u003eMagazine\u003c\/td\u003e\n\u003ctd\u003eCitations\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eThe Journal of Clinical Investigation\u003c\/td\u003e\n\u003ctd\u003eCerebrospinal fluid–based kinetic biomarkers of axonal transport in monitoring neurodegeneration\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"http:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC3428100\/\" target=\"_blank\"\u003ePubMed: PMC3428100\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eNeurobiology of Disease\u003c\/td\u003e\n\u003ctd\u003eLysosomal dysfunction increases exosome-mediated alpha-synuclein release and transmission\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"http:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC3107939\/\" target=\"_blank\"\u003ePubMed: PMC3107939\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eNature Communications\u003c\/td\u003e\n\u003ctd\u003eParkinson's disease induced pluripotent stem cells with triplication of the α-synuclein locus\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"http:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC3265381\/\" target=\"_blank\"\u003ePubMed: PMC3265381\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProvisional chapter\u003c\/td\u003e\n\u003ctd\u003eMutations of PARK Genes and Alpha-Synuclein and Parkin Concentrations in Parkinson’s Disease\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"http:\/\/cdn.intechopen.com\/pdfs-wm\/46430.pdf\" target=\"_blank\"\u003eIntechopen:Source\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eNerodegeneration\u003c\/td\u003e\n\u003ctd\u003eApolipoprotein Eε4: A Biomarker for Executive Dysfunction among Parkinson's Disease Patients with Mild Cognitive Impairment\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29326545\" target=\"_blank\"\u003epubmed:29326545\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eExperimental gerontology\u003c\/td\u003e\n\u003ctd\u003eThe anti-aging protein klotho alleviates injury of nigrostriatal dopaminergic pathway in 6-hydroxydopamine rat model of Parkinson's disease: Involvement of PKA\/CaMKII\/CREB signaling.\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29107062\" target=\"_blank\"\u003epubmed:29107062\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eNeurobiology of Disease\u003c\/td\u003e\n\u003ctd\u003eDevelopment and biochemical characterization of a mouse model of Parkinson's disease bearing defective glucocerebrosidase activity\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0969996118307563\" target=\"_blank\"\u003ePubmed: 30521842\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003emolecular and cellular biochemistry\u003c\/td\u003e\n\u003ctd\u003ePotential therapeutic effects of antagonizing adenosine A2A receptor, curcumin and niacin in rotenone-induced Parkinson's disease mice model\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/31820278\/\" target=\"_blank\"\u003ePubmed: 31820278\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eInternational Journal of Pharmacology\u003c\/td\u003e\n\u003ctd\u003eNose to brain delivery of rotigotine loaded chitosan nanoparticles in human SH-SY5Y neuroblastoma cells and animal model of Parkinson's disease\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32084576\/\" target=\"_blank\"\u003ePubmed: 32084576\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEBioMedicine\u003c\/td\u003e\n\u003ctd\u003eAnti-α-synuclein ASO delivered to monoamine neurons prevents α-synuclein accumulation in a Parkinson's disease-like mouse model and in monkeys\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32810825\/\" target=\"_blank\"\u003ePubmed: 32810825\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"product_relation\"\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr class=\"first_tr\"\u003e\n\u003ctd class=\"td_1\"\u003eCatalog No.\u003c\/td\u003e\n\u003ctd class=\"td_2\"\u003eRelated products for research use of Homo sapiens (Human) Organism species\u003c\/td\u003e\n\u003ctd class=\"td_3\"\u003eApplications (RESEARCH USE ONLY!)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRPB222Hu01\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/RPB222Hu01.html\"\u003eRecombinant Synuclein Alpha (SNCa)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003ePositive Control; Immunogen; SDS-PAGE; WB.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePAB222Hu01\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/PAB222Hu01.html\"\u003ePolyclonal Antibody to Synuclein Alpha (SNCa)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eWB; IHC; ICC; IP.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLAB222Hu71\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/LAB222Hu71.html\"\u003eBiotin-Linked Polyclonal Antibody to Synuclein Alpha (SNCa)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eWB; IHC; ICC.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMAB222Hu22\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/MAB222Hu22.html\"\u003eMonoclonal Antibody to Synuclein Alpha (SNCa)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eWB; IHC; ICC; IP.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSEB222Hu\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/SEB222Hu.html\"\u003eELISA Kit for Synuclein Alpha (SNCa)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eEnzyme-linked immunosorbent assay for Antigen Detection.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAEB222Hu\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/AEB222Hu.html\"\u003eELISA Kit for Anti-Synuclein Alpha Antibody (Anti-SNCa)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eEnzyme-linked immunosorbent assay for Antibody Detection.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Cloud-clone","offers":[{"title":"96T \/ The minimum detectable dose of this kit is typically less than 6.8pg\/mL \/ 15.6-1,000pg\/mL","offer_id":47592842559768,"sku":"SEB222Hu","price":665.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/SE_BIG_d55ec065-3d73-4866-ba4e-6bb8c2db6907.jpg?v=1706252331","url":"https:\/\/danabiosci.com\/products\/new-product-642016","provider":"Dana Bioscience","version":"1.0","type":"link"}