Cloud-clone
SKU:RPA215Hu01
Recombinant Collagen Type I Alpha 2 (COL1a2)
Recombinant Collagen Type I Alpha 2 (COL1a2)
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Prokaryotic expression
Product No.
RPA215Hu01
Organism Species
Homo sapiens (Human).
Source
Prokaryotic expression
Host
E.coli
Endotoxin Level
<1.0EU per 1µg (determined by the LAL method)
Subcellular Location
Secreted, Extracellular matrix
Predicted Molecular Mass
33.4kDa
Accurate Molecular Mass
33kDa(Analysis of differences refer to the manual)
Residues & Tags
Val1103~Lys1366 with N-terminal His Tag
Buffer Formulation
PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
Traits
Freeze-dried powder
Purity
> 97%
Isoelectric Point
5.4
Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
UOM
10µg 50µg 200µg 1mg 5mg
SEQUENCE
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Plos one | Inhibition of TGF-β Signaling Enables Human Corneal Endothelial Cell Expansion In Vitro for Use in Regenerative Medicine PubMed: PMC3581499 |
| J Proteomics | Characterization of secretomes from a human blood brain barrier endothelial cells in-vitro model after ischemia by stable isotope labeling with aminoacids in cell culture (SILAC) PubMed: 26718731 |
| PLoS One | Pro-fibrotic compounds induce stellate cell activation, ECM-remodelling and Nrf2 activation in a human 3D-multicellular model of liver fibrosis pubmed:28665955 |
| American Journal of Translational Research | A novel hybrid 3D-printed titanium scaffold for osteogenesis in a rabbit calvarial defect model Pubmed:29511441 |
| Sci Rep | The effect of hypoxia on the proteomic signature of pig adipose-derived stromal/stem cells (pASCs) Pubmed: 33208768 |
| Experimental and Therapeutic Medicine | Protective effects of halophyte complex extract against UVB?induced damage in human keratinocytes and the skin of hairless mice 33986847 |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| RPA215Hu01 | Recombinant Collagen Type I Alpha 2 (COL1a2) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA215Hu01 | Polyclonal Antibody to Collagen Type I Alpha 2 (COL1a2) | WB; ICC; IHC-P; IHC-F; ELISA. |
| MAA215Hu22 | Monoclonal Antibody to Collagen Type I Alpha 2 (COL1a2) | WB; IHC; ICC; IP. |
| SEA215Hu | ELISA Kit for Collagen Type I Alpha 2 (COL1a2) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA215Hu | Magnetic Luminex Assay Kit for Collagen Type I Alpha 2 (COL1a2) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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