{"product_id":"new-product-620522","title":"Active Interleukin 28B (IL28B)","description":"\u003cp data-mce-fragment=\"1\"\u003eProduct No.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eAPC028Hu01\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eOrganism Species\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eHomo sapiens (Human).\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eBuffer Formulation\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eTraits\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eFreeze-dried powder\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003ePurity\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u0026gt; 95%\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eIsoelectric Point\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e9.0\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eApplications\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eCell culture; Activity Assays.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eUOM\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e10µg 50µg 200µg 1mg 5mg\u003c\/p\u003e\n\u003cdiv class=\"product_left\"\u003e\n\u003ch3\u003eACTIVITY TEST\u003c\/h3\u003e\n\u003cdiv\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cdiv class=\"product_image\"\u003e\n\u003cimg src=\"https:\/\/www.cloud-clone.com\/upload\/product\/pictures\/APC028Hu01-201801111710123865.jpg\"\u003e\n\u003cp\u003eFigure. The binding activity of IL28B with IL10Rb.\u003cbr\u003eInterleukin 28B (IL28B) is a cytokine distantly related to type I interferons and the IL-10 family. It has function on antiviral, antitumour and immunomodulatory activities. IL28B also plays a critical role in the antiviral host defense, predominantly in the epithelial tissues and acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK\/STAT signaling pathway resulting in the expression of IFN-stimulated genes (ISG), which mediate the antiviral state. Besides, Interleukin 10 Receptor Beta (IL10Rb) has been identified as an interactor of IL28B, thus a binding ELISA assay was conducted to detect the interaction of recombinant human IL28B and recombinant human IL10Rb. Briefly, IL28B were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL10Rb-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IL28B pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IL28B and IL10Rb was shown in Figure 1, and this effect was in a dose dependent manner.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch3\u003eUSAGE\u003c\/h3\u003e\n\u003cdiv\u003e\n\u003cp\u003eReconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg\/mL. Do not vortex.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch3\u003eSTORAGE\u003c\/h3\u003e\n\u003cdiv\u003e\n\u003cp\u003eAvoid repeated freeze\/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch3\u003eSTABILITY\u003c\/h3\u003e\n\u003cdiv\u003e\n\u003cp\u003eThe thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"product_reference\"\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr class=\"first_tr\"\u003e\n\u003ctd class=\"td_1\"\u003eMagazine\u003c\/td\u003e\n\u003ctd\u003eCitations\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eJournal of Antimicrobial Chemotherapy\u003c\/td\u003e\n\u003ctd\u003eImpact of IL28B gene polymorphisms on interferon-λ3 plasma levels during pegylated interferon-α\/ribavirin therapy for chronic hepatitis C in patients coinfected with HIV\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/22294646\" target=\"_blank\"\u003ePubMed: 22294646\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCytokine\u003c\/td\u003e\n\u003ctd\u003eDifferent effects of three interferons L on Toll-like receptor-related gene expression in HepG2 cells.\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/24041672\" target=\"_blank\"\u003ePubmed: 24041672\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHepatology\u003c\/td\u003e\n\u003ctd\u003ePolymorphisms in melanoma differentiation‐associated gene 5 link protein function to clearance of hepatitis C virus\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/25130193\" target=\"_blank\"\u003ePubmed:25130193\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCytokine.\u003c\/td\u003e\n\u003ctd\u003eIncreased concentration of interferon lambda-3, interferon beta and interleukin-10 in the cerebrospinal fluid of patients with tick-borne encephalitis\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/25461389\" target=\"_blank\"\u003ePubmed:25461389\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eJ Biomed Res. \u003c\/td\u003e\n\u003ctd\u003eImpact of IL28B gene polymorphisms rs8099917 and rs12980275 on response to pegylated interferon-α\/ribavirin therapy in chronic hepatitis C genotype 4 …\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"http:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC4726832\/\" target=\"_blank\"\u003epmc:PMC4726832\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eJournal of Biomedical Research\u003c\/td\u003e\n\u003ctd\u003eImpact of IL28B gene polymorphisms rs8099917 and rs12980275 on response to pegylated interferon-α\/ribavirin therapy in chronic hepatitis C genotype 4 patients\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC4726832\/\" target=\"_blank\"\u003ePMC4726832\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLUPUS\u003c\/td\u003e\n\u003ctd\u003eCollagen triple helix repeat containing-1: a novel biomarker associated with disease activity in Systemic lupus erythematosus\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"http:\/\/journals.sagepub.com\/doi\/abs\/10.1177\/0961203318804877\" target=\"_blank\"\u003ePubmed: 30336754\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"product_relation\"\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr class=\"first_tr\"\u003e\n\u003ctd class=\"td_1\"\u003eCatalog No.\u003c\/td\u003e\n\u003ctd class=\"td_2\"\u003eRelated products for research use of Homo sapiens (Human) Organism species\u003c\/td\u003e\n\u003ctd class=\"td_3\"\u003eApplications (RESEARCH USE ONLY!)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAPC028Hu01\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/APC028Hu01.html\"\u003eActive Interleukin 28B (IL28B)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eCell culture; Activity Assays.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRPC028Hu01\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/RPC028Hu01.html\"\u003eRecombinant Interleukin 28B (IL28B)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003ePositive Control; Immunogen; SDS-PAGE; WB.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLAC028Hu81\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/LAC028Hu81.html\"\u003eFITC-Linked Polyclonal Antibody to Interleukin 28B (IL28B)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eWB; IHC; ICC; IF.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLAC028Hu71\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/LAC028Hu71.html\"\u003eBiotin-Linked Polyclonal Antibody to Interleukin 28B (IL28B)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eWB; IHC; ICC.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMAC028Hu22\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/MAC028Hu22.html\"\u003eMonoclonal Antibody to Interleukin 28B (IL28B)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eWB; IHC; ICC; IP.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHEC028Hu\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/HEC028Hu.html\"\u003eHigh Sensitive ELISA Kit for Interleukin 28B (IL28B)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eEnzyme-linked immunosorbent assay for Antigen Detection.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSEC028Hu\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/SEC028Hu.html\"\u003eELISA Kit for Interleukin 28B (IL28B)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eEnzyme-linked immunosorbent assay for Antigen Detection.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSCC028Hu\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/SCC028Hu.html\"\u003eCLIA Kit for Interleukin 28B (IL28B)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eChemiluminescent immunoassay for Antigen Detection.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLMC028Hu\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/LMC028Hu.html\"\u003eMagnetic Luminex Assay Kit for Interleukin 28B (IL28B) ,etc.\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eMagnetic Luminex Assay for Antigen Detection.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e","brand":"Cloud-clone","offers":[{"title":"50ug \/ \u003e 95% \/ Homo sapiens (Human)","offer_id":47569121902872,"sku":"APC028Hu01","price":528.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/NP_fe39939b-0d9d-4098-8b69-7b1cbcd1a192.jpg?v=1705984022","url":"https:\/\/danabiosci.com\/products\/new-product-620522","provider":"Dana Bioscience","version":"1.0","type":"link"}