{"product_id":"new-product-620452","title":"Active Fibroblast Growth Factor 3 (FGF3)","description":"\u003cp data-mce-fragment=\"1\"\u003eProduct No.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eAPC906Hu01\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eOrganism Species\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eHomo sapiens (Human).\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eBuffer Formulation\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e100mMNaHCO3, 500mMNaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eTraits\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eFreeze-dried powder\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003ePurity\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u0026gt; 90%\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eIsoelectric Point\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e11.0\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eApplications\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eCell culture; Activity Assays.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eUOM\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e10µg 50µg 200µg 1mg 5mg\u003c\/p\u003e\n\u003cdiv class=\"product_left\"\u003e\n\u003ch3\u003eACTIVITY TEST\u003c\/h3\u003e\n\u003cdiv\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cdiv class=\"product_image\"\u003e\n\u003cimg src=\"https:\/\/www.cloud-clone.com\/upload\/product\/pictures\/APC906Hu01-201811120924326703.png\"\u003e\n\u003cp\u003eFibroblast Growth Factor 3 (FGF3) also known as INT-2 proto-oncogene protein is a member of the fibroblast growth factor family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. Besides, Fibroblast Growth Factor Receptor 2 (FGFR2) has been identified as an interactor of FGF3, thus a binding ELISA assay was conducted to detect the interaction of recombinant human FGF3 and recombinant human FGFR2. Briefly, FGF3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to FGFR2-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-Fibroblast Growth Factor Receptor 2 (FGFR2) pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of FGF3 and FGFR2 was shown in Figure 1, and this effect was in a dose dependent manner.\u003cbr\u003eFigure. The binding activity of FGF3 with FGFR2.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch3\u003eUSAGE\u003c\/h3\u003e\n\u003cdiv\u003e\n\u003cp\u003eReconstitute in ddH2O to a concentration of 0.1-1.0 mg\/mL. Do not vortex.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch3\u003eSTORAGE\u003c\/h3\u003e\n\u003cdiv\u003e\n\u003cp\u003eAvoid repeated freeze\/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch3\u003eSTABILITY\u003c\/h3\u003e\n\u003cdiv\u003e\n\u003cp\u003eThe thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch3\u003e \u003c\/h3\u003e\n\u003cdiv\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"product_reference\"\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr class=\"first_tr\"\u003e\n\u003ctd class=\"td_1\"\u003eMagazine\u003c\/td\u003e\n\u003ctd\u003eCitations\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMol Med Rep\u003c\/td\u003e\n\u003ctd\u003eScreening a novel FGF3 antagonist peptide with anti-tumor effects on breast cancer from a phage display library\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/%2026323695\" target=\"_blank\"\u003ePubMed: 26323695\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"product_relation\"\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr class=\"first_tr\"\u003e\n\u003ctd class=\"td_1\"\u003eCatalog No.\u003c\/td\u003e\n\u003ctd class=\"td_2\"\u003eRelated products for research use of Homo sapiens (Human) Organism species\u003c\/td\u003e\n\u003ctd class=\"td_3\"\u003eApplications (RESEARCH USE ONLY!)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRPC906Hu01\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/RPC906Hu01.html\"\u003eRecombinant Fibroblast Growth Factor 3 (FGF3)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003ePositive Control; Immunogen; SDS-PAGE; WB.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAPC906Hu01\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/APC906Hu01.html\"\u003eActive Fibroblast Growth Factor 3 (FGF3)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eCell culture; Activity Assays.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRPC906Hu02\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/RPC906Hu02.html\"\u003eRecombinant Fibroblast Growth Factor 3 (FGF3)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003ePositive Control; Immunogen; SDS-PAGE; WB.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePAC906Hu01\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/PAC906Hu01.html\"\u003ePolyclonal Antibody to Fibroblast Growth Factor 3 (FGF3)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eWB; IHC; ICC; IP.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLAC906Hu71\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/LAC906Hu71.html\"\u003eBiotin-Linked Polyclonal Antibody to Fibroblast Growth Factor 3 (FGF3)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eWB; IHC; ICC.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMAC906Hu22\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/MAC906Hu22.html\"\u003eMonoclonal Antibody to Fibroblast Growth Factor 3 (FGF3)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eWB; IHC; ICC; IP.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSEC906Hu\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/SEC906Hu.html\"\u003eELISA Kit for Fibroblast Growth Factor 3 (FGF3)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eEnzyme-linked immunosorbent assay for Antigen Detection.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSCC906Hu\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/SCC906Hu.html\"\u003eCLIA Kit for Fibroblast Growth Factor 3 (FGF3)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eChemiluminescent immunoassay for Antigen Detection.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLMC906Hu\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/LMC906Hu.html\"\u003eMagnetic Luminex Assay Kit for Fibroblast Growth Factor 3 (FGF3) ,etc.\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eMagnetic Luminex Assay for Antigen Detection.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e","brand":"Cloud-clone","offers":[{"title":"50ug \/ \u003e 90% \/ Homo sapiens (Human)","offer_id":47569112432920,"sku":"APC906Hu01","price":528.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/NP_084ac1a3-be6f-4937-9c3e-4e42b4be383b.jpg?v=1705983745","url":"https:\/\/danabiosci.com\/products\/new-product-620452","provider":"Dana Bioscience","version":"1.0","type":"link"}