{"product_id":"new-product-620197","title":"Active Galectin 9 (GAL9)","description":"\u003cp\u003eProduct No.\u003c\/p\u003e\n\u003cp\u003eAPA309Hu01\u003c\/p\u003e\n\u003cp\u003eOrganism Species\u003c\/p\u003e\n\u003cp\u003eHomo sapiens (Human).\u003c\/p\u003e\n\u003cp\u003eBuffer Formulation\u003c\/p\u003e\n\u003cp\u003ePBS, pH7.4, containing 0.01% SKL, 5% Trehalose.\u003c\/p\u003e\n\u003cp\u003eTraits\u003c\/p\u003e\n\u003cp\u003eFreeze-dried powder\u003c\/p\u003e\n\u003cp\u003ePurity\u003c\/p\u003e\n\u003cp\u003e\u0026gt; 90%\u003c\/p\u003e\n\u003cp\u003eIsoelectric Point\u003c\/p\u003e\n\u003cp\u003e8.2\u003c\/p\u003e\n\u003cp\u003eApplications\u003c\/p\u003e\n\u003cp\u003eCell culture; Activity Assays.\u003c\/p\u003e\n\u003cp\u003eUOM\u003c\/p\u003e\n\u003cp\u003e10µg 50µg 200µg 1mg 5mg\u003c\/p\u003e\n\u003cdiv class=\"product\"\u003e\n\u003cdiv class=\"product_left\"\u003e\n\u003ch3\u003eACTIVITY TEST\u003c\/h3\u003e\n\u003cdiv\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cdiv class=\"product_image\"\u003e\n\u003cimg src=\"https:\/\/www.cloud-clone.com\/upload\/product\/pictures\/APA309Hu01-201612011552134695.jpg\"\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"product_image\"\u003e\n\u003cimg src=\"https:\/\/www.cloud-clone.com\/upload\/product\/pictures\/APA309Hu01-201612011552451497.jpg\"\u003e\n\u003cp\u003eGAL9 (Galectin-9) belongs to the galectin family, which is defined by their binding specificity for β-galactoside sugars, such as N-acetyllactosamine (Galβ1-3GlcNAc or Galβ1-4GlcNAc). It is reported that GAL9 induces T-helper type 1 lymphocyte (Th1) death by binding to HAVCR2 (Hepatitis A virus cellular receptor 2); besides, the interaction between GAL9 and PDI (Protein disulfide-isomerase) leads to disulfide reductase activity increasing at the plasma membrane, therefore alters the plasma membrane redox state and enhances cell migration. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human GAL9 with recombinant human HAVCR2 and recombinant human PDI separately. Briefly, GAL9 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to HAVCR2-coated and PDI-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-GAL9 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of GAL9 with HAVCR2 and PDI were shown in Figure 1 and Figure 2, and this effect was in a dose dependent manner.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch3\u003eUSAGE\u003c\/h3\u003e\n\u003cdiv\u003e\n\u003cp\u003eReconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg\/mL. Do not vortex.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch3\u003eSTORAGE\u003c\/h3\u003e\n\u003cdiv\u003e\n\u003cp\u003eAvoid repeated freeze\/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch3\u003eSTABILITY\u003c\/h3\u003e\n\u003cdiv\u003e\n\u003cp\u003eThe thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"product_reference\"\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr class=\"first_tr\"\u003e\n\u003ctd class=\"td_1\"\u003eMagazine\u003c\/td\u003e\n\u003ctd\u003eCitations\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAmerican Journal of Nephrology\u003c\/td\u003e\n\u003ctd\u003eTemporal Increases in Urinary Carboxymethyllysine Correlate with Albuminuria Development in Diabetes\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"http:\/\/content.karger.com\/ProdukteDB\/produkte.asp?Doi=328581\" target=\"_blank\"\u003eKarger: 328581\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eClinical Immunology\u003c\/td\u003e\n\u003ctd\u003eGalectin-9 administration ameliorates CVB3 induced myocarditis by promoting the proliferation of regulatory T cells and alternatively activated Th2 cells\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"http:\/\/www.sciencedirect.com\/science\/article\/pii\/S1521661611001057\" target=\"_blank\"\u003eScienceDirect: S1521661611001057\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRenal Failure\u003c\/td\u003e\n\u003ctd\u003eRat Renal Interstitial Fibroblasts Affect the Th1\/Th2 Profile In Vitro\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/22013937\" target=\"_blank\"\u003ePubmed: 22013937\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBMC Nephrol\u003c\/td\u003e\n\u003ctd\u003eSerum galectin-9 levels are elevated in the patients with type 2 diabetes and chronic kidney disease\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/23339460\" target=\"_blank\"\u003ePubMed: 23339460\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRetrovirology\u003c\/td\u003e\n\u003ctd\u003eDysregulated Tim-3 expression on natural killer cells is associated with increased Galectin-9 levels in HIV-1 infection\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"http:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC3750478\/\" target=\"_blank\"\u003ePubMed: PMC3750478\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePLoS One.\u003c\/td\u003e\n\u003ctd\u003eUpregulation of the Tim-3\/Galectin-9 Pathway of T Cell Exhaustion in Chronic Hepatitis B Virus Infection\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"http:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC3480425\/\" target=\"_blank\"\u003ePubMed: PMC3480425\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePLoS Pathogens\u003c\/td\u003e\n\u003ctd\u003eGalectin-9 and IL-21 Mediate Cross-regulation between Th17 and Treg Cells during Acute Hepatitis C\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"http:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC3688567\/\" target=\"_blank\"\u003ePubMed: PMC3688567\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePLOS ONE\u003c\/td\u003e\n\u003ctd\u003eGraves’ Disease Is Associated with a Defective Expression of the Immune Regulatory Molecule Galectin-9 in Antigen-Presenting Dendritic Cells\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/25880730\" target=\"_blank\"\u003ePubMed: 25880730\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdvances in Experimental Medicine and Biology\u003c\/td\u003e\n\u003ctd\u003eMolecules of Damage-Associated Patterns in Bronchoalveolar Lavage Fluid and Serum in Chronic Obstructive Pulmonary Disease\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29429028\" target=\"_blank\"\u003ePubmed:29429028\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eJournal of Microbiology, Immunology and Infection\u003c\/td\u003e\n\u003ctd\u003eGalectin-9-like from Angiostrongylus cantonensis young adult worms modulates eosinophil chemotaxis in vitro\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S1684118218304237\" target=\"_blank\"\u003eDoi: 10.1016\/j.jmii.2018.09.010\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOncology Letters\u003c\/td\u003e\n\u003ctd\u003eThe effects of Tim‑3 activation on T‑cells in gastric cancer progression\u003ca rel=\"nofollow\" href=\"https:\/\/www.spandidos-publications.com\/10.3892\/ol.2018.9743\" target=\"_blank\"\u003e\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eScientific Reports\u003c\/td\u003e\n\u003ctd\u003eCirculating levels of PD-L1 and Galectin-9 are associated with patient survival in surgically treated Hepatocellular Carcinoma independent of their intra …\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"https:\/\/www.nature.com\/articles\/s41598-019-47235-z\" target=\"_blank\"\u003ePubmed: 31337865\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiomolecules\u003c\/td\u003e\n\u003ctd\u003eBlood Levels of Galectin-9, an Immuno-Regulating Molecule, Reflects the Severity for the Acute and Chronic Infectious Diseases. Biomolecules 2021, 11, 430\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/33804076\" target=\"_blank\"\u003e33804076\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMedicine\u003c\/td\u003e\n\u003ctd\u003eRegulatory T cells, damage-associated molecular patterns, and myeloid-derived suppressor cells in bronchoalveolar lavage fluid interlinked with chronic obstructive …\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/35687771\/\" target=\"_blank\"\u003ePubmed:35687771\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"product_relation\"\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr class=\"first_tr\"\u003e\n\u003ctd class=\"td_1\"\u003eCatalog No.\u003c\/td\u003e\n\u003ctd class=\"td_2\"\u003eRelated products for research use of Homo sapiens (Human) Organism species\u003c\/td\u003e\n\u003ctd class=\"td_3\"\u003eApplications (RESEARCH USE ONLY!)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRPA309Hu01\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/RPA309Hu01.html\"\u003eRecombinant Galectin 9 (GAL9)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003ePositive Control; Immunogen; SDS-PAGE; WB.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAPA309Hu01\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/APA309Hu01.html\"\u003eActive Galectin 9 (GAL9)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eCell culture; Activity Assays.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAPA309Hu61\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/APA309Hu61.html\"\u003eActive Galectin 9 (GAL9)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eCell culture; Activity Assays.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEPA309Hu61\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/EPA309Hu61.html\"\u003eEukaryotic Galectin 9 (GAL9)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003ePositive Control; Immunogen; SDS-PAGE; WB.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePAA309Hu01\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/PAA309Hu01.html\"\u003ePolyclonal Antibody to Galectin 9 (GAL9)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eWB; IHC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMAA309Hu22\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/MAA309Hu22.html\"\u003eMonoclonal Antibody to Galectin 9 (GAL9)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eWB; IHC; ICC; IP.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSEA309Hu\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/SEA309Hu.html\"\u003eELISA Kit for Galectin 9 (GAL9)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eEnzyme-linked immunosorbent assay for Antigen Detection.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSCA309Hu\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/SCA309Hu.html\"\u003eCLIA Kit for Galectin 9 (GAL9)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eChemiluminescent immunoassay for Antigen Detection.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLMA309Hu\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/LMA309Hu.html\"\u003eMagnetic Luminex Assay Kit for Galectin 9 (GAL9) ,etc.\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eMagnetic Luminex Assay for Antigen Detection.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv id=\"more_info\"\u003e\u003c\/div\u003e","brand":"Cloud-clone","offers":[{"title":"50ug \/ \u003e 90% \/ Homo sapiens (Human)","offer_id":47569063706904,"sku":"APA309Hu01","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/NP_1197eb62-d09a-4510-bec5-73798e663565.jpg?v=1705982474","url":"https:\/\/danabiosci.com\/products\/new-product-620197","provider":"Dana Bioscience","version":"1.0","type":"link"}