{"product_id":"new-product-620180","title":"Active Carbonic Anhydrase II (CA2)","description":"\u003cp\u003eProduct No.\u003c\/p\u003e\n\u003cp\u003eAPA782Ra01\u003c\/p\u003e\n\u003cp\u003eOrganism Species\u003c\/p\u003e\n\u003cp\u003eRattus norvegicus (Rat).\u003c\/p\u003e\n\u003cp\u003eBuffer Formulation\u003c\/p\u003e\n\u003cp\u003e20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.\u003c\/p\u003e\n\u003cp\u003eTraits\u003c\/p\u003e\n\u003cp\u003eFreeze-dried powder\u003c\/p\u003e\n\u003cp\u003ePurity\u003c\/p\u003e\n\u003cp\u003e\u0026gt; 97%\u003c\/p\u003e\n\u003cp\u003eIsoelectric Point\u003c\/p\u003e\n\u003cp\u003e6.9\u003c\/p\u003e\n\u003cp\u003eApplications\u003c\/p\u003e\n\u003cp\u003eCell culture; Activity Assays.\u003c\/p\u003e\n\u003cp\u003eUOM\u003c\/p\u003e\n\u003cp\u003e10µg 50µg 200µg 1mg 5mg\u003c\/p\u003e\n\u003cdiv class=\"product\"\u003e\n\u003cdiv class=\"product_left\"\u003e\n\u003ch3\u003eACTIVITY TEST\u003c\/h3\u003e\n\u003cdiv\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cdiv class=\"product_image\"\u003e\n\u003cimg src=\"https:\/\/www.cloud-clone.com\/upload\/product\/pictures\/APA782Ra01-201701091102188345.jpg\" data-mce-src=\"https:\/\/www.cloud-clone.com\/upload\/product\/pictures\/APA782Ra01-201701091102188345.jpg\"\u003e\n\u003cp\u003eCA2 (Carbonic anhydrase 2) is an enzyme that catalyzes reversible hydration of carbon dioxide. It is essential for bone resorption and osteoclast differentiation and contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption. It is widely accepted that CA2 also catalyzes hydrolysis of p-Nitrophenyl Acetate. Thus, a hydration assay was conducted to test the catalytic activity of CA2 using 4-Nitrophenyl Acetate (4-NPA) as substrate. Briefly, different concentrations of CA2 were incubated with 1mM 4-NPA in reaction buffer. The absorbance at the wavelength of 400nm was read per hour, and the result was shown in figure 1. It is obvious that CA2 catalyzes hydrolysis of p-Nitrophenyl Acetate.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch3\u003eUSAGE\u003c\/h3\u003e\n\u003cdiv\u003e\n\u003cp\u003eReconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg\/mL. Do not vortex.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch3\u003eSTORAGE\u003c\/h3\u003e\n\u003cdiv\u003e\n\u003cp\u003eAvoid repeated freeze\/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch3\u003eSTABILITY\u003c\/h3\u003e\n\u003cdiv\u003e\n\u003cp\u003eThe thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"product_reference\"\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr class=\"first_tr\"\u003e\n\u003ctd class=\"td_1\"\u003eMagazine\u003c\/td\u003e\n\u003ctd\u003eCitations\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProteomics Clin Appl. \u003c\/td\u003e\n\u003ctd\u003eIdentification of potential breast cancer markers in nipple discharge by protein profile analysis using two-dimensional nano-liquid chromatography\/nanoelectrospray ionization-mass spectrometry.\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/26970563\" rel=\"nofollow\" data-mce-href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/26970563\" target=\"_blank\"\u003ePubmed:26970563\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePROTEOMICS - Clinical Applications\u003c\/td\u003e\n\u003ctd\u003eComparative mass spectrometric and immunoassay-based proteome analysis in serum ofDuchenne muscular dystrophy patients.\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/26680509\" rel=\"nofollow\" data-mce-href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/26680509\" target=\"_blank\"\u003epubmed:26680509\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCell \u0026amp; Molecular Biology\u003c\/td\u003e\n\u003ctd\u003eIdentification of potential breast cancer markers in nipple discharge by protein profile analysis using two‐dimensional nano‐liquid chromatography\/nanoelectrospray …\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"http:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/prca.201500016\/full\" rel=\"nofollow\" data-mce-href=\"http:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/prca.201500016\/full\" target=\"_blank\"\u003edoi:10.1002\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"product_relation\"\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr class=\"first_tr\"\u003e\n\u003ctd class=\"td_1\"\u003eCatalog No.\u003c\/td\u003e\n\u003ctd class=\"td_2\"\u003eRelated products for research use of Rattus norvegicus (Rat) Organism species\u003c\/td\u003e\n\u003ctd class=\"td_3\"\u003eApplications (RESEARCH USE ONLY!)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eNPA782Ra01\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/NPA782Ra01.html\" data-mce-href=\"https:\/\/www.cloud-clone.com\/products\/NPA782Ra01.html\"\u003eNative Carbonic Anhydrase II (CA2)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003ePositive Control; Immunogen; SDS-PAGE; WB.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAPA782Ra01\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/APA782Ra01.html\" data-mce-href=\"https:\/\/www.cloud-clone.com\/products\/APA782Ra01.html\"\u003eActive Carbonic Anhydrase II (CA2)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eCell culture; Activity Assays.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRPA782Ra01\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/RPA782Ra01.html\" data-mce-href=\"https:\/\/www.cloud-clone.com\/products\/RPA782Ra01.html\"\u003eRecombinant Carbonic Anhydrase II (CA2)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003ePositive Control; Immunogen; SDS-PAGE; WB.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePAA782Ra02\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/PAA782Ra02.html\" data-mce-href=\"https:\/\/www.cloud-clone.com\/products\/PAA782Ra02.html\"\u003ePolyclonal Antibody to Carbonic Anhydrase II (CA2)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eWB; IHC; ICC; IP.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePAA782Ra01\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/PAA782Ra01.html\" data-mce-href=\"https:\/\/www.cloud-clone.com\/products\/PAA782Ra01.html\"\u003ePolyclonal Antibody to Carbonic Anhydrase II (CA2)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eWB; IHC; ICC; IP.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLAA782Ra71\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/LAA782Ra71.html\" data-mce-href=\"https:\/\/www.cloud-clone.com\/products\/LAA782Ra71.html\"\u003eBiotin-Linked Polyclonal Antibody to Carbonic Anhydrase II (CA2)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eWB; IHC; ICC.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSEA782Ra\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/SEA782Ra.html\" data-mce-href=\"https:\/\/www.cloud-clone.com\/products\/SEA782Ra.html\"\u003eELISA Kit for Carbonic Anhydrase II (CA2)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eEnzyme-linked immunosorbent assay for Antigen Detection.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLMA782Ra\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/LMA782Ra.html\" data-mce-href=\"https:\/\/www.cloud-clone.com\/products\/LMA782Ra.html\"\u003eMagnetic Luminex Assay Kit for Carbonic Anhydrase II (CA2) ,etc.\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eMagnetic Luminex Assay for Antigen Detection.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eKSA782Ra01\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/KSA782Ra01.html\" data-mce-href=\"https:\/\/www.cloud-clone.com\/products\/KSA782Ra01.html\"\u003eELISA Kit DIY Materials for Carbonic Anhydrase II (CA2)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eMain materials for \"Do It (ELISA Kit) Yourself\".\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv id=\"more_info\"\u003e\u003c\/div\u003e","brand":"Cloud-clone","offers":[{"title":"50ug \/ \u003e 97% \/ Rattus norvegicus (Rat)","offer_id":47569061282072,"sku":"APA782Ra01","price":350.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/NP_cda6460d-2bd9-462f-ac6b-cadcf6ae59b2.jpg?v=1705982412","url":"https:\/\/danabiosci.com\/products\/new-product-620180","provider":"Dana Bioscience","version":"1.0","type":"link"}