{"product_id":"new-product-620110","title":"Active Ribonuclease P (RNASEP)","description":"\u003cp\u003e-\u003c\/p\u003e\n\u003cp\u003eProduct No.\u003c\/p\u003e\n\u003cp\u003eAPA198Ra01\u003c\/p\u003e\n\u003cp\u003eOrganism Species\u003c\/p\u003e\n\u003cp\u003eRattus norvegicus (Rat).\u003c\/p\u003e\n\u003cp\u003eBuffer Formulation\u003c\/p\u003e\n\u003cp\u003ePBS, pH7.4, containing 0.01% SKL, 5% Trehalose.\u003c\/p\u003e\n\u003cp\u003eTraits\u003c\/p\u003e\n\u003cp\u003eFreeze-dried powder\u003c\/p\u003e\n\u003cp\u003ePurity\u003c\/p\u003e\n\u003cp\u003e\u0026gt; 97%\u003c\/p\u003e\n\u003cp\u003eIsoelectric Point\u003c\/p\u003e\n\u003cp\u003e6.2\u003c\/p\u003e\n\u003cp\u003eApplications\u003c\/p\u003e\n\u003cp\u003eCell culture; Activity Assays.\u003c\/p\u003e\n\u003cp\u003eUOM\u003c\/p\u003e\n\u003cp\u003e10µg 50µg 200µg 1mg 5mg\u003c\/p\u003e\n\u003cdiv class=\"product\"\u003e\n\u003cdiv class=\"product_left\"\u003e\n\u003ch3\u003eACTIVITY TEST\u003c\/h3\u003e\n\u003cdiv\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cdiv class=\"product_image\"\u003e\n\u003cimg src=\"https:\/\/www.cloud-clone.com\/upload\/product\/pictures\/APA198Ra01-201704271549018443.jpg\"\u003e\n\u003cp\u003eRibonuclease P is a site specific endonuclease that generates mature tRNAs by catalysing the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'-terminus. It can also cleave other RNA substrates such as 4.5S RNA. In bacteria, RNase P consists of of two components: a large RNA (about 400 base pairs) encoded by rnpB, and a small protein (119 to 133 amino acids) encoded by rnpA. The RNA moiety of RNase P carries the catalytic activity; the protein component plays an auxiliary, but essential, role in vivo by binding to the 5'-leader sequence and broadening the substrate specificity of the ribozyme. The sequence of rnpA is not highly conserved, however there is, in the central part of the protein, a conserved basic region. Besides, Nucleophosmin (NPM) has been identified as an interactor of RNASEP, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat RNASEP and recombinant rat NPM. Briefly, RNASEP were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to NPM-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-RNASEP pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of RNASEP and NPM was shown in Figure 1, and this effect was in a dose dependent manner.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch3\u003eUSAGE\u003c\/h3\u003e\n\u003cdiv\u003e\n\u003cp\u003eReconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg\/mL. Do not vortex.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch3\u003eSTORAGE\u003c\/h3\u003e\n\u003cdiv\u003e\n\u003cp\u003eAvoid repeated freeze\/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch3\u003eSTABILITY\u003c\/h3\u003e\n\u003cdiv\u003e\n\u003cp\u003eThe thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition. \u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"product_relation\"\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr class=\"first_tr\"\u003e\n\u003ctd class=\"td_1\"\u003eCatalog No.\u003c\/td\u003e\n\u003ctd class=\"td_2\"\u003eRelated products for research use of Rattus norvegicus (Rat) Organism species\u003c\/td\u003e\n\u003ctd class=\"td_3\"\u003eApplications (RESEARCH USE ONLY!)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRPA198Ra01\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/RPA198Ra01.html\"\u003eRecombinant Ribonuclease P (RNASEP)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003ePositive Control; Immunogen; SDS-PAGE; WB.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAPA198Ra01\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/APA198Ra01.html\"\u003eActive Ribonuclease P (RNASEP)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eCell culture; Activity Assays.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePAA198Ra01\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/PAA198Ra01.html\"\u003ePolyclonal Antibody to Ribonuclease P (RNASEP)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eWB; IHC; ICC; IP.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv id=\"more_info\"\u003e\u003c\/div\u003e","brand":"Cloud-clone","offers":[{"title":"50ug \/ \u003e 97% \/ Rattus norvegicus (Rat)","offer_id":47569054957848,"sku":"APA198Ra01","price":770.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/NP_d78d43ed-b4b1-4fe4-b509-ab09bb9efae8.jpg?v=1705982155","url":"https:\/\/danabiosci.com\/products\/new-product-620110","provider":"Dana Bioscience","version":"1.0","type":"link"}