{"product_id":"new-product-619829","title":"Active Macrophage Inflammatory Protein 3 Alpha (MIP3a)","description":"\u003cp\u003eProduct No.\u003c\/p\u003e\n\u003cp\u003eAPA095Hu02\u003c\/p\u003e\n\u003cp\u003eOrganism Species\u003c\/p\u003e\n\u003cp\u003eHomo sapiens (Human).\u003c\/p\u003e\n\u003cp\u003eBuffer Formulation\u003c\/p\u003e\n\u003cp\u003e20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.\u003c\/p\u003e\n\u003cp\u003eTraits\u003c\/p\u003e\n\u003cp\u003eFreeze-dried powder\u003c\/p\u003e\n\u003cp\u003ePurity\u003c\/p\u003e\n\u003cp\u003e\u0026gt; 90%\u003c\/p\u003e\n\u003cp\u003eIsoelectric Point\u003c\/p\u003e\n\u003cp\u003e8.5\u003c\/p\u003e\n\u003cp\u003eApplications\u003c\/p\u003e\n\u003cp\u003eCell culture; Activity Assays.\u003c\/p\u003e\n\u003cp\u003eUOM\u003c\/p\u003e\n\u003cp\u003e10µg 50µg 200µg 1mg 5mg\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cdiv class=\"product\"\u003e\n\u003cdiv class=\"product_left\"\u003e\n\u003ch3\u003eACTIVITY TEST\u003c\/h3\u003e\n\u003cdiv\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cdiv class=\"product_image\"\u003e\n\u003cimg src=\"https:\/\/www.cloud-clone.com\/upload\/product\/pictures\/APA095Hu02-202305221634163180.jpg\"\u003e\n\u003cp\u003eMacrophage Inflammatory Protein 3 Alpha (MIP3a) also known as Chemokine (C-C motif) ligand 20 (CCL20) or liver activation regulated chemokine (LARC) is a small cytokine belonging to the CC chemokine family. It is strongly chemotactic for lymphocytes and weakly attracts neutrophils. MIP3a is implicated in the formation and function of mucosal lymphoid tissues via chemoattraction of lymphocytes and dendritic cells towards the epithelial cells surrounding these tissues. It is expressed in several tissues such as peripheral blood lymphocytes, lymph nodes, liver, appendix, fetal lung. Expression of MIP3a can be induced by microbial factors such as lipopolysaccharide (LPS), and inflammatory cytokines such as tumor necrosis factor and interferon-γ, and down-regulated by IL-10. Besides, lnterleukin 1 Alpha (IL1a) has been identified as an interactor of MIP3a, thus a binding ELISA assay was conducted to detect the interaction of recombinant human MIP3a and recombinant human IL1a. Briefly, MIP3a were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to IL1a-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-MIP3a pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450nm immediately. The binding activity of MIP3a and IL1a was shown in Figure 1, and this effect was in a dose dependent manner, the EC50 for this effect is 0.676 ug\/mL.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch3\u003eUSAGE\u003c\/h3\u003e\n\u003cdiv\u003e\n\u003cp\u003eReconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg\/mL. Do not vortex.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch3\u003eSTORAGE\u003c\/h3\u003e\n\u003cdiv\u003e\n\u003cp\u003eAvoid repeated freeze\/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch3\u003eSTABILITY\u003c\/h3\u003e\n\u003cdiv\u003e\n\u003cp\u003eThe thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"product_reference\"\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr class=\"first_tr\"\u003e\n\u003ctd class=\"td_1\"\u003eMagazine\u003c\/td\u003e\n\u003ctd\u003eCitations\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMedicine (Baltimore).\u003c\/td\u003e\n\u003ctd\u003eIncreased Serum Levels of Macrophage Inflammatory Protein-3α and Cystatin A Predict a Poor Prognosis of Nasopharyngeal Carcinoma\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/25396333\" target=\"_blank\"\u003ePubmed:25396333\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCancer\u003c\/td\u003e\n\u003ctd\u003eSerum chemokine network correlates with chemotherapy in non-small cell lung cancer\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/25976768\" target=\"_blank\"\u003ePubMed: 25976768\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAmerican Journal of Cancer Research\u003c\/td\u003e\n\u003ctd\u003eCCR6+ B lymphocytes responding to tumor cell-derived CCL20 support hepatocellular carcinoma progression via enhancing angiogenesis.\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/28560063\" target=\"_blank\"\u003epubmed:28560063\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiological Research\u003c\/td\u003e\n\u003ctd\u003eIn vitro chemokine (CC motif) receptor 6-dependent non-inflammatory chemotaxis during spermatogenesis\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"https:\/\/doi.org\/10.1186\/s40659-018-0161-z\" target=\"_blank\"\u003e10.1186:s40659-018-0161-z\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBioMed Research International\u003c\/td\u003e\n\u003ctd\u003eIndoleamine 2, 3-Dioxygenase (IDO) Regulates Th17\/Treg Immunity in Experimental IgA Nephropathy\u003ca rel=\"nofollow\" href=\"http:\/\/search.proquest.com\/openview\/76c734d03ac0c550115ec8136da936e1\/1?pq-origsite=gscholar\u0026amp;cbl=32484\" target=\"_blank\"\u003e\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"product_relation\"\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr class=\"first_tr\"\u003e\n\u003ctd class=\"td_1\"\u003eCatalog No.\u003c\/td\u003e\n\u003ctd class=\"td_2\"\u003eRelated products for research use of Homo sapiens (Human) Organism species\u003c\/td\u003e\n\u003ctd class=\"td_3\"\u003eApplications (RESEARCH USE ONLY!)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRPA095Hu01\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/RPA095Hu01.html\"\u003eRecombinant Macrophage Inflammatory Protein 3 Alpha (MIP3a)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003ePositive Control; Immunogen; SDS-PAGE; WB.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAPA095Hu01\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/APA095Hu01.html\"\u003eActive Macrophage Inflammatory Protein 3 Alpha (MIP3a)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eCell culture; Activity Assays.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRPA095Hu02\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/RPA095Hu02.html\"\u003eRecombinant Macrophage Inflammatory Protein 3 Alpha (MIP3a)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003ePositive Control; Immunogen; SDS-PAGE; WB.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAPA095Hu02\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/APA095Hu02.html\"\u003eActive Macrophage Inflammatory Protein 3 Alpha (MIP3a)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eCell culture; Activity Assays.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePAA095Hu01\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/PAA095Hu01.html\"\u003ePolyclonal Antibody to Macrophage Inflammatory Protein 3 Alpha (MIP3a)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eWB; IHC; ICC; IP.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePAA095Hu02\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/PAA095Hu02.html\"\u003ePolyclonal Antibody to Macrophage Inflammatory Protein 3 Alpha (MIP3a)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eWB; IHC; ICC; IP.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLAA095Hu71\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/LAA095Hu71.html\"\u003eBiotin-Linked Polyclonal Antibody to Macrophage Inflammatory Protein 3 Alpha (MIP3a)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eWB; IHC; ICC.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLAA095Hu81\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/LAA095Hu81.html\"\u003eFITC-Linked Polyclonal Antibody to Macrophage Inflammatory Protein 3 Alpha (MIP3a)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eWB; IHC; ICC; IF.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMAA095Hu22\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/MAA095Hu22.html\"\u003eMonoclonal Antibody to Macrophage Inflammatory Protein 3 Alpha (MIP3a)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eWB; IHC; ICC; IP.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMAA095Hu23\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/MAA095Hu23.html\"\u003eMonoclonal Antibody to Macrophage Inflammatory Protein 3 Alpha (MIP3a)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eWB; IHC; ICC; IP.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMAA095Hu24\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/MAA095Hu24.html\"\u003eMonoclonal Antibody to Macrophage Inflammatory Protein 3 Alpha (MIP3a)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eWB; IHC; ICC; IP.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMAA095Hu25\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/MAA095Hu25.html\"\u003eMonoclonal Antibody to Macrophage Inflammatory Protein 3 Alpha (MIP3a)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eWB; IHC; ICC; IP.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMAA095Hu27\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/MAA095Hu27.html\"\u003eMonoclonal Antibody to Macrophage Inflammatory Protein 3 Alpha (MIP3a)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eWB; IHC; ICC; IP.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMAA095Hu21\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/MAA095Hu21.html\"\u003eMonoclonal Antibody to Macrophage Inflammatory Protein 3 Alpha (MIP3a)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eWB,IHC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSEA095Hu\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/SEA095Hu.html\"\u003eELISA Kit for Macrophage Inflammatory Protein 3 Alpha (MIP3a)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eEnzyme-linked immunosorbent assay for Antigen Detection.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLMA095Hu\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/LMA095Hu.html\"\u003eMagnetic Luminex Assay Kit for Macrophage Inflammatory Protein 3 Alpha (MIP3a) ,etc.\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eMagnetic Luminex Assay for Antigen Detection.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eKSA095Hu01\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/KSA095Hu01.html\"\u003eELISA Kit DIY Materials for Macrophage Inflammatory Protein 3 Alpha (MIP3a)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eMain materials for \"Do It(ELISA Kit) Yourself\"\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv id=\"more_info\"\u003e\u003c\/div\u003e","brand":"Cloud-clone","offers":[{"title":"50ug \/ \u003e 90% \/ Homo sapiens (Human)","offer_id":47569029726488,"sku":"APA095Hu02","price":864.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/NP_d6978e70-0221-43bc-abe7-c6bb447de417.jpg?v=1705981073","url":"https:\/\/danabiosci.com\/products\/new-product-619829","provider":"Dana Bioscience","version":"1.0","type":"link"}