{"product_id":"new-product-617154","title":"Magnetic Luminex Assay Kit for Glucose Transporter 4 (GLUT4) ,etc.","description":"\u003cp data-mce-fragment=\"1\"\u003eProduct No.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eLMC023Ra\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eOrganism Species\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eRattus norvegicus (Rat).\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eSample Type\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eTissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eTest Method\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eDouble-antibody Sandwich\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eAssay Length\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e3.5h\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eDetection Range\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e0.01-10ng\/mL\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eSensitivity\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eThe minimum detectable dose of this kit is typically less than 0.003 ng\/mL.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eUOM\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003cdiv class=\"product\"\u003e\n\u003cdiv class=\"product_left\"\u003e\n\u003ch3\u003eSpecificity\u003c\/h3\u003e\n\u003cdiv\u003e\n\u003cp\u003eThis assay has high sensitivity and excellent specificity for detection of Glucose Transporter 4 (GLUT4) ,etc..\u003cbr\u003eNo significant cross-reactivity or interference between Glucose Transporter 4 (GLUT4) ,etc. and analogues was observed.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch3\u003eRecovery\u003c\/h3\u003e\n\u003cdiv\u003e\n\u003cp\u003eMatrices listed below were spiked with certain level of recombinant Glucose Transporter 4 (GLUT4) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Glucose Transporter 4 (GLUT4) ,etc. in samples.\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eMatrix\u003c\/td\u003e\n\u003ctd\u003eRecovery range (%)\u003c\/td\u003e\n\u003ctd\u003eAverage(%)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eserum(n=5)\u003c\/td\u003e\n\u003ctd\u003e83-97\u003c\/td\u003e\n\u003ctd\u003e89\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEDTA plasma(n=5)\u003c\/td\u003e\n\u003ctd\u003e94-105\u003c\/td\u003e\n\u003ctd\u003e101\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eheparin plasma(n=5)\u003c\/td\u003e\n\u003ctd\u003e88-96\u003c\/td\u003e\n\u003ctd\u003e91\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3\u003ePrecision\u003c\/h3\u003e\n\u003cdiv\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glucose Transporter 4 (GLUT4) ,etc. were tested 20 times on one plate, respectively.\u003cbr\u003eInter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glucose Transporter 4 (GLUT4) ,etc. were tested on 3 different plates, 8 replicates in each plate.\u003cbr\u003eCV(%) = SD\/meanX100\u003cbr\u003eIntra-Assay: CV\u0026lt;10%\u003cbr\u003eInter-Assay: CV\u0026lt;12%\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch3\u003eLinearity\u003c\/h3\u003e\n\u003cdiv\u003e\n\u003cp\u003eThe linearity of the kit was assayed by testing samples spiked with appropriate concentration of Glucose Transporter 4 (GLUT4) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample\u003c\/td\u003e\n\u003ctd\u003e1:2\u003c\/td\u003e\n\u003ctd\u003e1:4\u003c\/td\u003e\n\u003ctd\u003e1:8\u003c\/td\u003e\n\u003ctd\u003e1:16\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eserum(n=5)\u003c\/td\u003e\n\u003ctd\u003e81-97%\u003c\/td\u003e\n\u003ctd\u003e92-101%\u003c\/td\u003e\n\u003ctd\u003e92-105%\u003c\/td\u003e\n\u003ctd\u003e78-103%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEDTA plasma(n=5)\u003c\/td\u003e\n\u003ctd\u003e93-105%\u003c\/td\u003e\n\u003ctd\u003e87-101%\u003c\/td\u003e\n\u003ctd\u003e95-105%\u003c\/td\u003e\n\u003ctd\u003e78-96%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eheparin plasma(n=5)\u003c\/td\u003e\n\u003ctd\u003e98-105%\u003c\/td\u003e\n\u003ctd\u003e82-98%\u003c\/td\u003e\n\u003ctd\u003e88-102%\u003c\/td\u003e\n\u003ctd\u003e80-104%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3\u003eStability\u003c\/h3\u003e\n\u003cdiv\u003e\n\u003cp\u003eThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.\u003cbr\u003eTo minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch3\u003eReagents and materials provided\u003c\/h3\u003e\n\u003cdiv\u003e\n\u003cdiv class=\"reagent_div\"\u003e\n\u003ctable class=\"reagent_table\"\u003e\n\u003ctbody\u003e\n\u003ctr class=\"first_tr\"\u003e\n\u003ctd\u003eReagents\u003c\/td\u003e\n\u003ctd\u003eQuantity\u003c\/td\u003e\n\u003ctd\u003eReagents\u003c\/td\u003e\n\u003ctd\u003eQuantity\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e96-well plate\u003c\/td\u003e\n\u003ctd\u003e1\u003c\/td\u003e\n\u003ctd\u003ePlate sealer for 96 wells\u003c\/td\u003e\n\u003ctd\u003e4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePre-Mixed Standard\u003c\/td\u003e\n\u003ctd\u003e2\u003c\/td\u003e\n\u003ctd\u003eStandard Diluent\u003c\/td\u003e\n\u003ctd\u003e1×20mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePre-Mixed Magnetic beads (22#:GLUT4)\u003c\/td\u003e\n\u003ctd\u003e1\u003c\/td\u003e\n\u003ctd\u003eAnalysis buffer\u003c\/td\u003e\n\u003ctd\u003e1×20mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePre-Mixed Detection Reagent A\u003c\/td\u003e\n\u003ctd\u003e1×120μL\u003c\/td\u003e\n\u003ctd\u003eAssay Diluent A\u003c\/td\u003e\n\u003ctd\u003e1×12mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDetection Reagent B (PE-SA)\u003c\/td\u003e\n\u003ctd\u003e1×120μL\u003c\/td\u003e\n\u003ctd\u003eAssay Diluent B\u003c\/td\u003e\n\u003ctd\u003e1×12mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSheath Fluid\u003c\/td\u003e\n\u003ctd\u003e1×10mL\u003c\/td\u003e\n\u003ctd\u003eWash Buffer (30 × concentrate)\u003c\/td\u003e\n\u003ctd\u003e1×20mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eInstruction manual\u003c\/td\u003e\n\u003ctd\u003e1\u003c\/td\u003e\n\u003ctd\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch3\u003eAssay procedure summary\u003c\/h3\u003e\n\u003cdiv\u003e\n\u003cp\u003e1. Preparation of standards, reagents and samples before the experiment;\u003cbr\u003e2. Add 100μL standard or sample to each well,\u003cbr\u003e    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;\u003cbr\u003e3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;\u003cbr\u003e4. Wash plate on magnetic frame for three times;\u003cbr\u003e5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;\u003cbr\u003e6. Wash plate on magnetic frame for three times;\u003cbr\u003e7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"product_reference\"\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr class=\"first_tr\"\u003e\n\u003ctd class=\"td_1\"\u003eMagazine\u003c\/td\u003e\n\u003ctd\u003eCitations\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePolar Record\u003c\/td\u003e\n\u003ctd\u003eGlucose transporter-4 in white blood cells of young and old sled dogs: a model for human biomarker development\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"http:\/\/journals.cambridge.org\/action\/displayAbstract?fromPage=online\u0026amp;aid=9091911\" target=\"_blank\"\u003eCambridge: Source\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eThe International Journal of Biochemistry \u0026amp;amp; Cell Biology\u003c\/td\u003e\n\u003ctd\u003eConditioning causes an increase in glucose transporter-4 levels in mononuclear cells in sled dogs\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/25236492\" target=\"_blank\"\u003ePubmed:25236492\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePsychoneuroendocrinology\u003c\/td\u003e\n\u003ctd\u003eMaternal stress predicts altered biogenesis and the profile of mitochondrial proteins in the frontal cortex and hippocampus of adult offspring rats\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/%2026143539\" target=\"_blank\"\u003ePubMed: 26143539\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePLOS ONE\u003c\/td\u003e\n\u003ctd\u003eAntidiabetic Effect of Galantamine: Novel Effect for a Known Centrally Acting Drug\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/26262991\" target=\"_blank\"\u003ePubMed: 26262991\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAmerican Journal of Animal and Veterinary Sciences\u003c\/td\u003e\n\u003ctd\u003eMetabolic Features of Heart Failure with Different Etiology\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"http:\/\/thescipub.com\/PDF\/ofsp.11075.pdf\" target=\"_blank\"\u003eofsp11075\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eJournal of Pharmaceutical Negative Results\u003c\/td\u003e\n\u003ctd\u003eHibiscus sabdariffa linn fruits methanolic extract and fractions mediated glucose uptake stimulation and glucose transporter 4 regulation\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"http:\/\/www.pnrjournal.com\/article.asp?issn=0976-9234;year=2019;volume=10;issue=1;spage=25;epage=31;aulast=Shadhan\" target=\"_blank\"\u003eDoi: 10.4103\/jpnr.JPNR_14_18\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eeureka herba indonesia\u003c\/td\u003e\n\u003ctd\u003eEffect of Noni Fruit Extract (Morinda citrifolia) on Glucose Intake to Diabetes Mellitus White Rat Muscle Tissue\u003ca rel=\"nofollow\" href=\"https:\/\/eurekabiomedical.com\/index.php\/EHI\/article\/download\/2\/8\" target=\"_blank\"\u003e\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eeureka herba indonesia\u003c\/td\u003e\n\u003ctd\u003eThe Effects of Sambiloto Leaf Extract (Andrographis peniculata) on Blood Sugar Regulation: an In Vivo Study\u003ca rel=\"nofollow\" href=\"https:\/\/eurekabiomedical.com\/index.php\/EHI\/article\/download\/1\/6\" target=\"_blank\"\u003e\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eeureka herba indonesia\u003c\/td\u003e\n\u003ctd\u003eEffect of Bay Leaf Extract (Syzygium polyanthum) on Blood Sugar Regulation via GLUT4 Protein Regulation in White Rat Muscle Tissue Induced Aloxan\u003ca rel=\"nofollow\" href=\"https:\/\/eurekabiomedical.com\/index.php\/EHI\/article\/download\/4\/7\" target=\"_blank\"\u003e\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEureka Herba Indonesia\u003c\/td\u003e\n\u003ctd\u003ePotential Combination of Tinospora crispa, Andrographis paniculata, Cinamomum burmanii, Syzygium polyanthum and Momordica charantia Extracts Against?¡­\u003ca rel=\"nofollow\" href=\"https:\/\/eurekabiomedical.com\/index.php\/EHI\/article\/download\/14\/24\" target=\"_blank\"\u003e\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"product_relation\"\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr class=\"first_tr\"\u003e\n\u003ctd class=\"td_1\"\u003eCatalog No.\u003c\/td\u003e\n\u003ctd class=\"td_2\"\u003eRelated products for research use of Rattus norvegicus (Rat) Organism species\u003c\/td\u003e\n\u003ctd class=\"td_3\"\u003eApplications (RESEARCH USE ONLY!)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRPC023Ra01\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/RPC023Ra01.html\"\u003eRecombinant Glucose Transporter 4 (GLUT4)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003ePositive Control; Immunogen; SDS-PAGE; WB.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePAC023Ra01\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/PAC023Ra01.html\"\u003ePolyclonal Antibody to Glucose Transporter 4 (GLUT4)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eWB; IHC; ICC; IP.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMAC023Ra21\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/MAC023Ra21.html\"\u003eMonoclonal Antibody to Glucose Transporter 4 (GLUT4)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eWB; IHC; ICC; IP.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSEC023Ra\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/SEC023Ra.html\"\u003eELISA Kit for Glucose Transporter 4 (GLUT4)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eEnzyme-linked immunosorbent assay for Antigen Detection.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLMC023Ra\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/LMC023Ra.html\"\u003eMagnetic Luminex Assay Kit for Glucose Transporter 4 (GLUT4) ,etc.\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eMagnetic Luminex Assay for Antigen Detection.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eKSC023Ra01\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/KSC023Ra01.html\"\u003eELISA Kit DIY Materials for Glucose Transporter 4 (GLUT4)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eMain materials for \"Do It(ELISA Kit) Yourself\"\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv id=\"more_info\"\u003e\u003c\/div\u003e","brand":"Cloud-clone","offers":[{"title":"0.01-10ng\/mL \/ 96T \/ typically less than 0.003 ng\/mL","offer_id":47567939600664,"sku":"LMC023Ra","price":912.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/LMC023Ra_baa8faae-f71a-4f6f-bee3-4f81933fcc57.jpg?v=1705965168","url":"https:\/\/danabiosci.com\/products\/new-product-617154","provider":"Dana Bioscience","version":"1.0","type":"link"}