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SKU:LMC470Hu
Magnetic Luminex Assay Kit for Fatty Acid Synthase (FASN) ,etc.
Magnetic Luminex Assay Kit for Fatty Acid Synthase (FASN) ,etc.
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Product No.
LMC470Hu
Organism Species
Homo sapiens (Human).
Sample Type
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Test Method
Double-antibody Sandwich
Assay Length
3.5h
Detection Range
0.02-20ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.007 ng/mL.
UOM
1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex
Specificity
This assay has high sensitivity and excellent specificity for detection of Fatty Acid Synthase (FASN) ,etc..
No significant cross-reactivity or interference between Fatty Acid Synthase (FASN) ,etc. and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Fatty Acid Synthase (FASN) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Fatty Acid Synthase (FASN) ,etc. in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 78-97 | 93 |
| EDTA plasma(n=5) | 89-101 | 95 |
| heparin plasma(n=5) | 90-104 | 96 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fatty Acid Synthase (FASN) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fatty Acid Synthase (FASN) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Fatty Acid Synthase (FASN) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 78-88% | 98-105% | 93-105% | 95-104% |
| EDTA plasma(n=5) | 79-97% | 78-96% | 85-101% | 83-101% |
| heparin plasma(n=5) | 84-103% | 80-92% | 79-94% | 86-98% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:FASN) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
| Magazine | Citations |
| BMC Complementary and Alternative Medicine | Identification of plasma protein markers common to patients with malignant tumour and Abnormal Savda in Uighur medicine: a prospective clinical study Pubmed:25652121 |
| Oncogenesis. | Diet-induced alteration of fatty acid synthase in prostate cancer progression Pubmed:26878389 |
| Animal Science Journal | Proteomic analysis to unravel the effect of heat stress on gene expression and milk synthesis in bovine mammary epithelial cells pubmed:28804941 |
| life sciences | Clinical importance of FASN in relation to HIF-1α and SREBP-1c in gastric adenocarcinoma Pubmed: 30914315 |
| Nutrients | High-dose Glycerol Monolaurate Up-Regulated Beneficial Indigenous Microbiota without Inducing Metabolic Dysfunction and Systemic Inflammation: New Insights … |
| bmc research notes | The role of CA-125, GLS and FASN in predicting cytoreduction for epithelial ovarian cancers Pubmed: 32698888 |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| RPC470Hu02 | Recombinant Fatty Acid Synthase (FASN) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC470Hu01 | Recombinant Fatty Acid Synthase (FASN) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC470Hu02 | Polyclonal Antibody to Fatty Acid Synthase (FASN) | WB; IHC; ICC; IP. |
| PAC470Hu01 | Polyclonal Antibody to Fatty Acid Synthase (FASN) | WB; IHC; ICC; IP. |
| MAC470Hu22 | Monoclonal Antibody to Fatty Acid Synthase (FASN) | WB; IHC; ICC; IP. |
| SEC470Hu | ELISA Kit for Fatty Acid Synthase (FASN) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC470Hu | Magnetic Luminex Assay Kit for Fatty Acid Synthase (FASN) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
| KSC470Hu01 | ELISA Kit DIY Materials for Fatty Acid Synthase (FASN) | Main materials for "Do It(ELISA Kit) Yourself" |
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