Cloud-clone
SKU:LML507Mu
Magnetic Luminex Assay Kit for Family With Sequence Similarity 3, Member B (FAM3B) ,etc.
Magnetic Luminex Assay Kit for Family With Sequence Similarity 3, Member B (FAM3B) ,etc.
Couldn't load pickup availability
Product No.
LML507Mu
Organism Species
Mus musculus (Mouse).
Sample Type
Serum, plasma, tissue homogenates and other biological fluids.
Test Method
Double-antibody Sandwich
Assay Length
3.5h
Detection Range
0.02-20ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.007 ng/mL.
UOM
1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex
Specificity
This assay has high sensitivity and excellent specificity for detection of Family With Sequence Similarity 3, Member B (FAM3B) ,etc..
No significant cross-reactivity or interference between Family With Sequence Similarity 3, Member B (FAM3B) ,etc. and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Family With Sequence Similarity 3, Member B (FAM3B) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Family With Sequence Similarity 3, Member B (FAM3B) ,etc. in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 81-104 | 88 |
| EDTA plasma(n=5) | 79-102 | 94 |
| heparin plasma(n=5) | 79-105 | 92 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Family With Sequence Similarity 3, Member B (FAM3B) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Family With Sequence Similarity 3, Member B (FAM3B) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Family With Sequence Similarity 3, Member B (FAM3B) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 79-103% | 80-90% | 96-105% | 98-105% |
| EDTA plasma(n=5) | 85-101% | 98-105% | 91-102% | 83-99% |
| heparin plasma(n=5) | 78-96% | 79-101% | 88-98% | 98-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:FAM3B) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
| Magazine | Citations |
| Journal of Diabetes Investigation | Elevated circulating level of a cytokine, pancreatic-derived factor, is associated with metabolic syndrome components in a Chinese population Doi: 10.1111 |
| Molecular and Cellular Endocrinology | Pancreatic-derived factor impaired glucagon-like Peptide-1 production from GLUTag enterendorine L-cell line and intestines pubmed:28549991 |
| Scientific Reports | FAM3B mediates high glucose-induced vascular smooth muscle cell proliferation and migration via inhibition of miR-322-5p pubmed:28536423 |
| Journal of diabetes investigation | Effects of circulating FAM3B on the risk of developing metabolic syndrome and its components: a five‐year prospective study pubmed:29178453 |
| Pamukkale Tıp Dergisi | The evaluation of serum Pancreatic-derived factor and Malondialdehyde levels in patients with polycystic ovary syndrome |
| Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
| RPL507Mu01 | Recombinant Family With Sequence Similarity 3, Member B (FAM3B) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPL507Mu02 | Recombinant Family With Sequence Similarity 3, Member B (FAM3B) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAL507Mu02 | Polyclonal Antibody to Family With Sequence Similarity 3, Member B (FAM3B) | WB; IHC; ICC; IP. |
| PAL507Mu01 | Polyclonal Antibody to Family With Sequence Similarity 3, Member B (FAM3B) | WB; IHC; ICC; IP. |
| SEL507Mu | ELISA Kit for Family With Sequence Similarity 3, Member B (FAM3B) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LML507Mu | Magnetic Luminex Assay Kit for Family With Sequence Similarity 3, Member B (FAM3B) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
Share
