Recombinant Human RANTES

Product Name

Recombinant Human Rantes

Size

50μg, 200μg, 1mg

Species

Human

Host

E.Coli

Expression Region

24-91

Mol. Weight

28.5 kDa

Tags

N-terminal His-IF2DI Tag

Purity

Greater than 90% as determined by SDS-PAGE

Application

Western Blot, ELISA

Activity

Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR5. The ED50 for this effect is 10-30 ng/mL.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% trehalose, pH7.4

Reconstitution

Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex

Storage

The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months.Avoid repeated freeze/thaw cycle.

Alternative Names

Beta chemokine RANTES, Beta chemokine RANTES precursor, C C motif chemokine 5, CCL 5, CCL5, CCL5_HUMAN, Chemokine (C C motif) ligand 5, Chemokine CC Motif Ligand 5, D17S136E, EoCP, Eosinophil chemotactic cytokine, MGC17164, RANTES(4-68)