{"product_id":"new-product-596675","title":"Recombinant Human Macrophage Inflammatory1 alpha","description":"\u003cp\u003e\u003cstrong\u003eProduct Name\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eRecombinant Human Macrophage Inflammatory1 alpha\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e50μg, 200μg, 1mg\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eHuman\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eE.Coli\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eExpression Region\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e23-92\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eMol. Weight\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e7.6 kDa\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTags\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eN-terminal His Tag\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eGreater than 95% as determined by SDS-PAGE\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eWestern Blot, ELISA\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eActivity\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eMeasured by its ability to chemoattract 2-day cultured human monocytes. The ED50 for this effect is 5-20 ng\/mL\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFormulation\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eLyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eReconstitution\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eCentrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg\/ml by gently pipetting 2-3 times, don't vortex\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months.Avoid repeated freeze\/thaw cycle.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAlternative Names\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eC C motif chemokine 3, CCL 3, CCL3, CCL3_HUMAN, Chemokine (C C motif) ligand 3, Chemokine C C motif ligand 3, Chemokine ligand 3, G0\/G1 switch regulatory protein 19 1, G0\/G1 switch regulatory protein 19-1, G0S19 1, G0S19 1 protein, Heparin binding chemotaxis protein, L2G25B, LD78 alpha\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"FineTest","offers":[{"title":"23-92 \/ 7.6 kDa \/ 200ug","offer_id":47558646464792,"sku":"P4659","price":626.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/fine_test_logo_990edd3c-8031-4cc8-9059-4708b270c01b.jpg?v=1705805174","url":"https:\/\/danabiosci.com\/products\/new-product-596675","provider":"Dana Bioscience","version":"1.0","type":"link"}