Recombinant Human M-CSF

Product Name

Recombinant Human M-CSF

Size

50μg, 200μg, 1mg

Species

Human

Host

CHO cells

Expression Region

33-190

Mol. Weight

25 kDa

Tags

C-terminal His Tag

Purity

Greater than 90% as determined by SDS-PAGE

Application

Western Blot, ELISA

Activity

Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 2-10 ng/mL.

Formulation

Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4

Reconstitution

Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex

Storage

The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months.Avoid repeated freeze/thaw cycle.

Alternative Names

CSF 1, CSF1, Lanimostim, M CSF, MCSF, M-CSF