{"product_id":"new-product-596512","title":"Recombinant Mouse IGF1","description":"\u003cp\u003e\u003cstrong\u003eProduct Name\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eRecombinant Mouse IGF1\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e50μg, 200μg, 1mg\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eMouse\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eE.Coli\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eExpression Region\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e49-118\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eMol. Weight\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e28.3 kDa\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTags\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eN-terminal His-IF2DI Tag\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eGreater than 95% as determined by SDS-PAGE\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eWestern Blot, ELISA\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eActivity\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eMeasured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 for this effect is 1-2.6 ng\/mL.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFormulation\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eLyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eReconstitution\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eCentrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg\/ml by gently pipetting 2-3 times, don't vortex\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months.Avoid repeated freeze\/thaw cycle.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAlternative Names\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIBP1, IGF I, IGF IA, IGF IB, IGF-I, Igf1, IGF1_HUMAN, IGF1A, IGFI, IGFIA, Insulin like growth factor 1, Insulin like growth factor 1 (somatomedin C), Insulin like growth factor IA, Insulin like growth factor IB, Insulin-like growth factor I, Mechano growth factor, MGF\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"FineTest","offers":[{"title":"49-118 \/ 7.6 kDa \/ 200ug","offer_id":47558617301272,"sku":"P4496","price":870.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/fine_test_logo_24dd1c76-5a49-4725-adc4-f62f9759f52c.jpg?v=1705804518","url":"https:\/\/danabiosci.com\/products\/new-product-596512","provider":"Dana Bioscience","version":"1.0","type":"link"}