{"product_id":"new-product-596465","title":"Recombinant Human Fibronectin type III EDB","description":"\u003cp\u003e\u003cstrong\u003eProduct Name\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eRecombinant Human Fibronectin type III EDB\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e50μg, 200μg, 1mg\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eHuman\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eE.Coli\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eExpression Region\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1244-1377\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eMol. Weight\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e14.6 kDa\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTags\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eN-terminal His Tag\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eGreater than 95% as determined by SDS-PAGE\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eWestern Blot, ELISA\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFormulation\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eLyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eReconstitution\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eCentrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg\/ml by gently pipetting 2-3 times, don't vortex\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months.Avoid repeated freeze\/thaw cycle.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAlternative Names\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eCIG, Cold insoluble globulin, Cold-insoluble globulin, DKFZp686F10164, DKFZp686H0342, DKFZp686I1370, DKFZp686O13149, ED B, Fibronectin 1, FINC, FINC_HUMAN, FN, FN1, FNZ, GFND, GFND2, LETS, Migration stimulating factor, MSF, Ugl-Y3\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"FineTest","offers":[{"title":"1244-1377 \/ 14.6 kDa \/ 200ug","offer_id":47558614286616,"sku":"P4448","price":626.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/fine_test_logo_feca1ef6-ce0d-4357-826a-0c62616c33fb.jpg?v=1705804340","url":"https:\/\/danabiosci.com\/products\/new-product-596465","provider":"Dana Bioscience","version":"1.0","type":"link"}