FineTest
SKU:EM1940
Mouse Spink1 (Serine protease inhibitor Kazal-type 1)ELISA Kit
Mouse Spink1 (Serine protease inhibitor Kazal-type 1)ELISA Kit
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Product Name
Mouse Spink1 (Serine protease inhibitor Kazal-type 1)ELISA Kit
Alias
Serine protease inhibitor Kazal-type 1 ELISA Kit, P12 ELISA Kit, Prostatic secretory glycoprotein ELISA Kit, Serine protease inhibitor Kazal-type 3 ELISA Kit, Spink1 ELISA Kit, Spink3 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
Spink1 ELISA Kit allows for the in vitro quantitative determination of Spink1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.25-2000pg/ml
Sensitivity
< 18.75pg/ml
Species
Mouse
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of Spink1 and the recovery rates were calculated by comparing the measured value to the expected amount of Spink1 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
88-102 |
96 |
|
EDTA plasma(n=5) |
92-105 |
97 |
|
heparin plasma(n=5) |
85-100 |
95 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Spink1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
88-105% |
85-99% |
93-103% |
|
EDTA plasma(n=5) |
83-101% |
82-101% |
84-98% |
|
heparin plasma(n=5) |
80-97% |
85-92% |
81-95% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
