FineTest
SKU:EH11010
Human PEF1(Peflin) ELISA Kit
Human PEF1(Peflin) ELISA Kit
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Product Name
Human PEF1（Peflin） ELISA Kit
Alias
Peflin ELISA Kit, PEF protein with a long N-terminal hydrophobic domain ELISA Kit, Penta-EF hand domain-containing protein 1 ELISA Kit, ABP32 ELISA Kit, UNQ1845 ELISA Kit, PRO3573 ELISA Kit, PEF1 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
PEF1（Peflin） ELISA Kit allows for the in vitro quantitative determination of PEF1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.25-2000pg/ml
Sensitivity
< 18.75pg/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of PEF1 and the recovery rates were calculated by comparing the measured value to the expected amount of PEF1 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
90-104 |
98 |
|
EDTA plasma(n=5) |
90-105 |
99 |
|
heparin plasma(n=5) |
91-102 |
95 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PEF1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-103% |
98-104% |
86-95% |
|
EDTA plasma(n=5) |
85-99% |
83-100% |
88-101% |
|
heparin plasma(n=5) |
84-99% |
80-92% |
80-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
