FineTest
SKU:EH12912
Human TFAM(Transcription factor A, mitochondrial) ELISA Kit
Human TFAM(Transcription factor A, mitochondrial) ELISA Kit
Couldn't load pickup availability
Product Name
Human TFAM(Transcription factor A, mitochondrial) ELISA Kit
Alias
TFAM ELISA Kit, Transcription factor A, mitochondrial ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
TFAM ELISA Kit allows for the in vitro quantitative determination of TFAM concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.25-2000pg/ml
Sensitivity
< 18.75pg/ml
Species
Human
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of TFAM and the recovery rates were calculated by comparing the measured value to the expected amount of TFAM in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
89-101 |
96 |
|
EDTA plasma(n=5) |
85-98 |
93 |
|
heparin plasma(n=5) |
87-104 |
97 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TFAM and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
92-104% |
89-104% |
89-105% |
|
EDTA plasma(n=5) |
82-100% |
82-100% |
83-100% |
|
heparin plasma(n=5) |
82-98% |
81-97% |
82-95% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
