FineTest
SKU:EM1925
Mouse SPON2(Spondin-2) ELISA Kit
Mouse SPON2(Spondin-2) ELISA Kit
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Product Name
Mouse SPON2(Spondin-2) ELISA Kit
Alias
Spondin-2 ELISA Kit, Mindin ELISA Kit, Spon2 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
SPON2 ELISA Kit allows for the in vitro quantitative determination of SPON2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Mouse
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of SPON2 and the recovery rates were calculated by comparing the measured value to the expected amount of SPON2 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
88-103 |
95 |
|
EDTA plasma(n=5) |
90-102 |
95 |
|
heparin plasma(n=5) |
93-100 |
97 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SPON2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-99% |
91-102% |
88-95% |
|
EDTA plasma(n=5) |
82-98% |
86-100% |
83-91% |
|
heparin plasma(n=5) |
82-100% |
81-88% |
84-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
