FineTest
SKU:ER1931
Rat RF-IgG (Rheumatoid Factor IgG) ELISA Kit
Rat RF-IgG (Rheumatoid Factor IgG) ELISA Kit
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Product Name
Rat RF-IgG (Rheumatoid Factor IgG) ELISA Kit
Alias
Rheumatoid Factor IgG ELISA Kit, RF-IgG ELISA Kit
Detection Method
Indirect ELISA
Application
RF-IgG ELISA Kit allows for the in vitro quantitative determination of RF-IgG concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
3.125-200IU/ml
Sensitivity
< 1.875IU/ml
Species
Rat
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of RF-IgG and the recovery rates were calculated by comparing the measured value to the expected amount of RF-IgG in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
90-99 |
93 |
|
EDTA plasma(n=5) |
90-100 |
95 |
|
heparin plasma(n=5) |
89-102 |
95 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of RF-IgG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-104% |
86-105% |
90-105% |
|
EDTA plasma(n=5) |
82-101% |
83-101% |
84-98% |
|
heparin plasma(n=5) |
81-99% |
90-99% |
84-96% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
