FineTest
SKU:ER1932
Rat RF-IgM (Rheumatoid Factor IgM) ELISA Kit
Rat RF-IgM (Rheumatoid Factor IgM) ELISA Kit
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Product Name
Rat RF-IgM (Rheumatoid Factor IgM) ELISA Kit
Alias
RF-IgM ELISA Kit, Rheumatoid Factor IgM ELISA Kit
Detection Method
Indirect ELISA
Application
RF-IgM ELISA Kit allows for the in vitro quantitative determination of RF-IgM concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
4.688-300IU/ml
Sensitivity
< 2.813IU/ml
Species
Rat
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of RF-IgM and the recovery rates were calculated by comparing the measured value to the expected amount of RF-IgM in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
88-105 |
95 |
|
EDTA plasma(n=5) |
85-100 |
93 |
|
heparin plasma(n=5) |
86-97 |
94 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of RF-IgM and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
87-102% |
85-105% |
85-99% |
|
EDTA plasma(n=5) |
86-97% |
83-101% |
82-98% |
|
heparin plasma(n=5) |
80-86% |
85-99% |
85-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
