FineTest
SKU:EM1913
Mouse SFRP5 (Secreted frizzled-related protein 5) ELISA Kit
Mouse SFRP5 (Secreted frizzled-related protein 5) ELISA Kit
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Product Name
Mouse SFRP5 (Secreted frizzled-related protein 5) ELISA Kit
Alias
Secreted frizzled-related protein 5 ELISA Kit, sFRP-5 ELISA Kit, Sfrp5 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
SFRP5 ELISA Kit allows for the in vitro quantitative determination of SFRP5 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Mouse
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of SFRP5 and the recovery rates were calculated by comparing the measured value to the expected amount of SFRP5 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
88-105 |
97 |
|
EDTA plasma(n=5) |
85-98 |
90 |
|
heparin plasma(n=5) |
85-105 |
97 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SFRP5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
88-105% |
85-105% |
94-100% |
|
EDTA plasma(n=5) |
83-101% |
82-92% |
83-101% |
|
heparin plasma(n=5) |
88-99% |
83-100% |
80-98% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
