FineTest
SKU:EM1456
Mouse β-EP(Beta-Endorphin) ELISA Kit
Mouse β-EP(Beta-Endorphin) ELISA Kit
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Product Name
Mouse β-EP(Beta-Endorphin) ELISA Kit
Alias
β-EP ELISA Kit
Detection Method
Competitive ELISA, Coated with Antigen
Application
β-EP ELISA Kit allows for the in vitro quantitative determination of β-EP concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Mouse
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of β-EP and the recovery rates were calculated by comparing the measured value to the expected amount of β-EP in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-102 |
93 |
|
EDTA plasma(n=5) |
85-101 |
91 |
|
heparin plasma(n=5) |
86-105 |
93 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of β-EP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-98% |
86-101% |
85-97% |
|
EDTA plasma(n=5) |
84-101% |
83-95% |
82-95% |
|
heparin plasma(n=5) |
81-88% |
87-100% |
91-96% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
