FineTest
SKU:EH0511
Human PDGFA(Platelet-derived growth factor subunit A) ELISA Kit
Human PDGFA(Platelet-derived growth factor subunit A) ELISA Kit
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PDGFA|Platelet Derived Growth Factor Subunit A|PDGF-A
Product Name
Human PDGFA(Platelet-derived growth factor subunit A) ELISA Kit
Alias
PDGFA ELISA Kit, Platelet Derived Growth Factor Subunit A ELISA Kit, PDGF-A ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
PDGFA ELISA Kit allows for the in vitro quantitative determination of PDGFA concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of PDGFA and the recovery rates were calculated by comparing the measured value to the expected amount of PDGFA in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
86-103 |
95 |
|
EDTA plasma(n=5) |
91-103 |
96 |
|
heparin plasma(n=5) |
85-93 |
91 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PDGFA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-95% |
85-104% |
91-103% |
|
EDTA plasma(n=5) |
82-101% |
87-98% |
86-100% |
|
heparin plasma(n=5) |
84-98% |
83-98% |
81-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
