{"product_id":"new-product-585031","title":"anti- VPS35 antibody","description":"\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eProduct Name\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eVPS35 antibody\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSize\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e100µg\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eForm\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eliquid\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003ePurification\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eImmunogen affinity purified\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003ePurity\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e≥95% as determined by SDS-PAGE\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eHost\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eRabbit\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eClonality\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003epolyclonal\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIsotype\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eIgG\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003ePBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze \/ thaw cycles.)\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eBACKGROUND\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eActs as component of the retromer cargo-selective complex(CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3. The CSC seems to associate with the cytoplasmic domain of cargo proteins predominantly via VPS35; however, these interactions seem to be of low affinity and retromer SNX proteins may also contribute to cargo selectivity thus questioning the classical function of the CSC. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network(TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX3-retromer mediates the retrograde endosome-to-TGN transport of WLS distinct from the SNX-BAR retromer pathway. The SNX27-retromer is believed to be involved in endosome-to-plasma membrane trafficking and recycling of a broad spectrum of cargo proteins. The CSC seems to act as recruitment hub for other proteins, such as the WASH complex and TBC1D5(Probable). Required for retrograde transport of lysosomal enzyme receptor IGF2R and SLC11A2. Required to regulate transcytosis of the polymeric immunoglobulin receptor(pIgR-pIgA)(PubMed:15078903, PubMed:15247922, PubMed:20164305). Required for endosomal localization of FAM21C(PubMed:22070227). Mediates the association of the CSC with the WASH complex via FAM21(PubMed:22070227, PubMed:24980502, PubMed:24819384). Required for the endosomal localization of TBC1D5(PubMed:20923837).\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIMMUNOGEN INFORMATION\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eImmunogen\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003evacuolar protein sorting 35 homolog\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSynonyms\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eDKFZp434E1211, DKFZp434P1672, FLJ10752, FLJ13588, FLJ20388, hVPS35, Maternal embryonic 3, MEM3, Vesicle protein sorting 35, VPS35\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eObserved MW\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e92 kDa\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eAPPLICATION\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eTested Application\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eELISA, WB, IF, IP\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eRecommended Dilution\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eWB: 1:200-1:1000; IP: 1:200-1：1000; IF: 1：10-1：100\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eUNIPROT INFORMATION\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eUniProt ID\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003ca href=\"http:\/\/www.uniprot.org\/uniprot\/Q96QK1\" data-mce-fragment=\"1\" data-mce-href=\"http:\/\/www.uniprot.org\/uniprot\/Q96QK1\"\u003eQ96QK1\u003c\/a\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIMAGES\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003cfigure class=\"w-full md:w-2\/3 p-2 border relative\"\u003e\u003cimg class=\"mx-auto\" alt=\"\" src=\"https:\/\/www.fn-test.com\/content\/uploads\/product\/images\/antibody\/image2\/FNab09439.jpg\"\u003e\u003c\/figure\u003e\n\u003cdiv class=\"w-full md:pl-8 pt-4 md:pt-0 md:w-1\/3\"\u003eImmunofluorescent analysis of HepG2 cells using FNab09439(VPS35 Antibody) at dilution of 1:25.\u003c\/div\u003e\n\u003cfigure class=\"w-full md:w-2\/3 p-2 border relative\"\u003e\u003cimg class=\"mx-auto\" alt=\"\" src=\"https:\/\/www.fn-test.com\/content\/uploads\/product\/images\/antibody\/image3\/FNab09439.jpg\"\u003e\u003c\/figure\u003e\n\u003cdiv class=\"w-full md:pl-8 pt-4 md:pt-0 md:w-1\/3\"\u003eIP Result of anti-VPS35 (IP:FNab09439, 4ug; Detection:FNab09439 1:600) with mouse kidney tissue lysate 4000ug.\u003c\/div\u003e\n\u003cfigure class=\"w-full md:w-2\/3 p-2 border relative\"\u003e\u003cimg class=\"mx-auto\" alt=\"\" src=\"https:\/\/www.fn-test.com\/content\/uploads\/product\/images\/antibody\/image4\/FNab09439.jpg\"\u003e\u003c\/figure\u003e\n\u003cdiv class=\"w-full md:pl-8 pt-4 md:pt-0 md:w-1\/3\"\u003eHepG2 cells were subjected to SDS PAGE followed by western blot with FNab09439(VPS35 Antibody) at dilution of 1:600\u003c\/div\u003e\n\u003cbr\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"FineTest","offers":[{"title":"100µg \/ Rabbit \/ polyclonal","offer_id":47549822042392,"sku":"FNab09439","price":260.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/fine_test_logo_0180d966-d3cc-4251-81bf-4871b5010c92.jpg?v=1705724305","url":"https:\/\/danabiosci.com\/products\/new-product-585031","provider":"Dana Bioscience","version":"1.0","type":"link"}