FineTest
SKU:ER1999
Rat OXB(Orexin B) ELISA Kit
Rat OXB(Orexin B) ELISA Kit
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Product Name
Rat OXB (Orexin B) ELISA Kit
Alias
OXB ELISA Kit, Orexin B ELISA Kit
Detection Method
Competitive ELISA, Coated with Antigen
Application
OXB ELISA Kit allows for the in vitro quantitative determination of OXB concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
62.5-4000pg/ml
Sensitivity
< 37.5pg/ml
Species
Rat
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of OXB and the recovery rates were calculated by comparing the measured value to the expected amount of OXB in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
81-101 |
90 |
|
EDTA plasma(n=5) |
85-104 |
94 |
|
heparin plasma(n=5) |
89-104 |
98 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of OXB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
84-97% |
80-99% |
81-98% |
|
EDTA plasma(n=5) |
90-97% |
92-100% |
90-101% |
|
heparin plasma(n=5) |
90-97% |
91-98% |
90-98% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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