{"product_id":"new-product-568860","title":"Ksr-1 Polyclonal Antibody","description":"\u003cp\u003eKSR1 (kinase supressor of Ras) was identified from a genetic screen in Drosophila and C. elegans as a component of the Ras signaling pathway. KSR1 has a putative carboxy-terminal kinase domain that lacks a key Lys residue for phospho-group transfer. Although reports indicate that ceramide and EGF activate KSR1, other evidence demonstrates that KSR1 regulates Raf in a kinase-independent manner. It is now widely accepted that KSR1 functions as a scaffold that binds MEK1\/2 and 14-3-3 protein constitutively and binds ERK1\/2 in a Ras activation-dependent manner. HSP70\/HSP90 and p50 Cdc37 associate with the KSR1 complex to ensure its stability. Multiple phosphorylation sites have been identified: ser297 and Ser392 mediate 14-3-3 binding, and putative MAPK phosphorylation sites include Thr260, Thr274 and Ser443. C-TAK1 (Cdc25C-associated kinase 1) binds and phosphorylates KSR1 at Ser392 in quiescent cells. In response to stimuli, Ser392 is dephosphorylated by PP2A, which leads to ERK1\/2 association and allows the KSR1 complex to translocate from cytosol to membrane, where the MAPK pathway is activated. IMP, a Ras-responsive E3 ubiquitin ligase, is also involved in interaction with KSR1 and may regulate its localization and stability. Very high expression levels of KSR1 inhibit MAPK signaling, whereas physiological levels promote MAPK signaling, indicating that the scaffold protein can turn signaling \"on\" or \"off\" depending on the scaffold concentration.\u003c\/p\u003e\n\u003cp\u003eThe Ksr-1 Polyclonal Antibody is a highly specific and reliable tool for the detection and analysis of Ksr-1 protein expression in various biological samples. This antibody has been meticulously developed and validated to ensure exceptional performance and accuracy in a wide range of applications, including Western blotting, immunohistochemistry, and immunofluorescence.\u003cbr\u003e\u003cbr\u003eThe Ksr-1 Polyclonal Antibody exhibits a high affinity and selectivity towards Ksr-1, a critical component of the MAPK signaling pathway. By targeting Ksr-1, this antibody enables researchers to investigate the role of this protein in cellular processes such as cell proliferation, differentiation, and survival.\u003cbr\u003e\u003cbr\u003eWith its exceptional sensitivity, the Ksr-1 Polyclonal Antibody allows for the detection of even low levels of Ksr-1 expression, facilitating precise quantification and analysis. Its robust performance ensures reliable and reproducible results, making it an invaluable tool for both basic research and clinical studies.\u003cbr\u003e\u003cbr\u003eThis antibody has been rigorously tested and validated using a variety of experimental approaches, ensuring its specificity and reliability. It exhibits minimal cross-reactivity with other proteins, guaranteeing accurate and specific detection of Ksr-1.\u003cbr\u003e\u003cbr\u003eThe Ksr-1 Polyclonal Antibody is supplied as a ready-to-use solution, eliminating the need for time-consuming and error-prone antibody preparation. Its user-friendly format allows for easy and convenient handling, saving valuable time in the laboratory.\u003cbr\u003e\u003cbr\u003eIn summary, the Ksr-1 Polyclonal Antibody is a highly specific, sensitive, and reliable tool for the detection and analysis of Ksr-1 protein expression. Its exceptional performance and accuracy make it an indispensable asset for researchers in various fields, enabling in-depth investigations into the role of Ksr-1 in cellular processes and disease mechanisms.\u003c\/p\u003e","brand":"BT Lab","offers":[{"title":"100ul \/ Rabbit \/ Primary antibody","offer_id":47542048522520,"sku":"BT-AP04882","price":275.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/BT_Lab_9a6df8ae-f6da-4c2d-8d1d-d381e0406e29.png?v=1705627137","url":"https:\/\/danabiosci.com\/products\/new-product-568860","provider":"Dana Bioscience","version":"1.0","type":"link"}