Dbf4 Polyclonal Antibody

The Dbf4/Cdc7 protein kinase is essential for the activation of replication origins during S phase. Cdc7-Dbf4 efficiently phosphorylates several proteins that are required for the initiation of DNA replication, including five of the six minichromosome maintenance (Mcm) proteins and the p180 subunit of DNA polymerase α-primase. This protein complex consists of the catalytic subunit Cdc7 associating with the regulatory and activating subunit Dbf4, and the kinase activity of the complex is regulated throughout the cell cycle mainly by fluctuating levels of Dbf4. Cdc7 is consistently expressed throughout the cell cycle, while the expression of Dbf4 is absent during G1 phase and accumulates during S and G2 phases. The anaphase-promoting complex rapidly degrades Dbf4 at the time of chromosome segregation, and the stability of Dbf4 remains low during pre-Start G1 phase. The coordinated degradation of Dbf4 and the time of chromosomes separation is important to ensuring that prereplicative complexes, which assemble after chromosome segregation, do not immediately refire.

The Dbf4 Polyclonal Antibody is a highly specific and reliable tool designed for the detection and analysis of Dbf4 protein in various biological samples. This antibody has been extensively validated and exhibits exceptional sensitivity and specificity, ensuring accurate and reproducible results.

The Dbf4 Polyclonal Antibody is produced using state-of-the-art techniques, guaranteeing high purity and batch-to-batch consistency. It has been rigorously tested for cross-reactivity with other proteins to ensure minimal interference and reliable performance.

This antibody is suitable for a wide range of applications, including Western blotting, immunohistochemistry, and immunofluorescence. It is compatible with various sample types, including cell lysates, tissue extracts, and purified proteins.

The Dbf4 Polyclonal Antibody offers exceptional performance in both research and clinical settings. Its robustness and reliability make it an indispensable tool for studying the function and regulation of Dbf4 protein in various biological processes.

With its superior quality and performance, the Dbf4 Polyclonal Antibody is an essential addition to any laboratory or research facility. It provides researchers with a valuable tool for advancing their understanding of Dbf4 protein and its role in cellular processes.